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Myc tag Mouse mAb - Primary antibody, specific to Myc, Mouse IgG1

COA COA
    Application:
  • IF/ICC
  • IP
  • WB
  • Host species: Mouse
  • Reactive species: Species independent
  • Isotype: Mouse IgG1
  • Clone Number: 7D9-4C6-6F10
  • Conjugation: Unconjugated
In stock
Item Number
Ab116621
Grouped product items
SKU Size
Availability
 Price Qty
Ab116621-10μl
10μl
1
$49.90
Ab116621-50μl
50μl
4
$149.90
Ab116621-100μl
100μl
3
$209.90
Ab116621-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,599.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (7D9-4C6-6F10); Mouse anti Myc tag Antibody; WB, ICC, IF, IP; Unconjugated

View related series
Myc tag (4)

Basic Description

Product Name Myc tag Mouse mAb - Primary antibody, specific to Myc, Mouse IgG1
Synonyms c-myc tag antibody | Myc Epitope Tag antibody | avian myelocytomatosis viral oncogene homolog | BHLHE39 | bHLHe39MRTL | Class E basic helix-loop-helix protein 39 | cMyc | c-Myc | myc proto-oncogene protein | Myc | Myc2 | MYCC | myc-related translation/loc
Specifications & Purity ExactAb™, Validated, High performance, 1.0 mg/mL
Host species Mouse
Specificity Myc
Immunogen A synthetic peptide derived from Myc-Tag (EQKLISEEDL).
Positive Control WB: 11654-myc lysates. ICC/IF: 293 cells transfected with a Myc tag protein. IP: Transfected 293 cell lysates with c-Myc tag fusion protein
Conjugation Unconjugated
Grade ExactAb™, High Performance, Validated
Product Description

Mouse anti Myc tag Antibody, Monoclonal (7D9-4C6-6F10), could be used for WB, ICC, IF, IP and so on.
Application:
WB: 1/1000 - 1/10000
IF/ICC: 1/100 - 1/500
IP: 1/200
Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein鈥檚 biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Product Properties

Isotype Mouse IgG1
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Myc tag Mouse mAb (Ab116621) - Western Blot
All lanes: Myc tag Mouse mAb (Ab116621) at 1/5000 dilution
Samples: Lysates at 5 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution

Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 0.1 s

Myc tag Mouse mAb (Ab116621) - IP
Immunoprecipitation analysis of c-Myc Tag in
1: Untransfected HEK293 cell lysate
2: Transfected HEK293 cell lysate with c-Myc tag fusion protein
3: IP (transfected HEK293 + normal mouse IgG+Protein G agarose)
4: IP (transfected HEK293 + Myc tag Mouse mAb (Ab116621) + Protein G agarose)

Application

Application Dilution info
WB

1/1000 - 1/10000

IP

1/200

IF/ICC

1/100-1/500

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1101598 Certificate of Analysis Nov 08, 2023 Ab116621
ZJ23F1101599 Certificate of Analysis Nov 08, 2023 Ab116621

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