Basic Description

Product Name	Mre11 Mouse mAb - Primary antibody, specific to MRE11, IgG2b
Synonyms	AT like diseaseantibody \| Ataxia telangiectasia disorder like antibody \| ATLD antibody \| DNA recombination and repair proteinantibody \| Double strand break repair protein MRE11A antibody \| Double-strand break repair protein MRE11A antibody \| endo/exonucle
Specifications & Purity	ExactAb™, Validated, Carrier Free, 1.0mg/mL
Host species	Mouse
Specificity	MRE11
Immunogen	Purified recombinant fragment of human MRE 11 (AA: 182-582) expressed in E. Coli.
Positive Control	WB: Hela, A431, MCF-7, Jurkat, HepG2, K562, COS-7, PC-12 and NIH/3T3 cell lysate. IHC: Ovarian cancer tissues; Rectal cancer tissues; Mouse testis tissues; Rat testis tissues; Rabbit testis tissues. Flow: Hela cells; K562 cells.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human Mre11 Antibody, Monoclonal antibody, could be used for ELISA, WB, Flow, IHC and so on. Application: WB: 1/500 - 1/2000. Predicted molecular weight: 80.6 kDa. IHC: 1/200 - 1/1000 Flow: 1/200 - 1/400 ELISA: 1/10000 Protein Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. Post-translational Phosphorylated upon DNA damage, probably by ATM or ATR.

Product Properties

Isotype	IgG2b
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Shape	Liquid
Concentration	1.0mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Associated Targets(Human)

MRE11 Tbio Double-strand break repair protein MRE11 (0 Activities)

Discover Target: MRE11

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: Mre11 Mouse mAb (Ab115912)

Mre11 Mouse mAb (Ab115912) - Western Blot
All lanes: Mre11 Mouse mAb (Ab115912) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 80.6 kDa
Observed band size: 84.2 kDa

Flow Cytometry: Mre11 Mouse mAb (Ab115912)

Mre11 Mouse mAb (Ab115912) - Flow Cytometry
Flow cytometric analysis of cell line labeling Mre11 with Mre11 Mouse mAb (Ab115912) (green) compared with a negative control (red).

Flow Cytometry: Mre11 Mouse mAb (Ab115912)

Mre11 Mouse mAb (Ab115912) - Flow Cytometry
Flow cytometric analysis of K562 cell line labeling Mre11 with Mre11 Mouse mAb (Ab115912) (green) compared with a negative control (red).