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RNA related
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AlkB (RNA/DNA demethylase, Nuclease-free)Species: Escherichia coli (strain K12) Accession #: P05050.1Expression system: E. coliBioactivity: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
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T4 RNA Ligase 2, truncatedSpecies: Enterobacteria phage T4 Accession #: P32277.1Expression system: E. coliBioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 10 µl in 1 hour at 25℃.Synonyms: T4 Rnl2, truncated | Rnl2 (1-249） | RNA ligase 2 | rnlB
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T4 RNA Ligase 2, truncated KQSpecies: Enterobacteria phage T4 Accession #: P32277.1Expression system: E. coliBioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.Synonyms: T4 Rnl2tr KQ | T4 Rnl2tr R55K, K227Q
