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RNA related
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AlkB (RNA/DNA demethylase, Nuclease-free)Species: Escherichia coli (strain K12) Accession #: P05050.1Expression system: E. coliBioactivity: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
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RNase III (dsRNA-specific)Species: Shigella sonnei Accession #: B1XB41.1Expression system: E. coliBioactivity: One unit is the amount of enzyme required to digest 1µg of dsRNA to siRNA in 20 minutes at 37ºC in a total reaction volume of 50µl.Synonyms: Ribonuclease III (dsRNA specific)
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Thermostable RNase T1Cas Number: 9026-12-4 EC Number: 232-809-1Formula: Molecular Weight: 11 kDaSynonyms: Thermostable Ribonuclease T1 | Ribonuclease T1
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Poly(U) PolymeraseSpecies: Schizosaccharomyces pombe Accession #: O13833.2Expression system: E. coliBioactivity: One unit is defined as the amount of enzyme that incorporates 1nmol of UMP into RNA in a 50μl volume in 10 minutes at 37ºC.Synonyms: PolyU Polymerase | PUP
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T4 RNA Ligase 2, truncatedSpecies: Enterobacteria phage T4 Accession #: P32277.1Expression system: E. coliBioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 10 µl in 1 hour at 25℃.Synonyms: T4 Rnl2, truncated | Rnl2 (1-249） | RNA ligase 2 | rnlB
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T4 RNA Ligase 2, truncated KQSpecies: Enterobacteria phage T4 Accession #: P32277.1Expression system: E. coliBioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.Synonyms: T4 Rnl2tr KQ | T4 Rnl2tr R55K, K227Q
