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Gene Editing
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AlkB (RNA/DNA demethylase, Nuclease-free)Species: Escherichia coli (strain K12) Accession #: P05050.1Expression system: E. coliBioactivity: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
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Lambda ExonucleaseSynonyms: λ Exonuclease | exo | lambdap24
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SP6 RNA PolymeraseAccession #: Q7Y5N4Expression system: E. coliBioactivity: One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
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T7 Endonuclease IAccession #: P00641.1Expression system: E. coliBioactivity: One unit is defined as the amount of enzyme required to convert > 90% of 1μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37℃.
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Thermolabile dsDNaseAccession #: C9YSL6Expression system: Pichia pastoris
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Cas9 Nuclease (SpCas9)Accession #: Q99ZW2.1Expression system: E. coliSynonyms: CRISPR-associated endonuclease Cas9 | Csn1
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Cas9 NLS (SpCas9-NLS)Accession #: Q99ZW2.1Expression system: E. coliSynonyms: CRISPR-associated endonuclease Cas9 | Csn1 | cas9
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T3 RNA PolymeraseAccession #: YP_009792932.1,P07659Expression system: E. coliBioactivity: One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
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Vaccinia DNA Topoisomerase ISpecies: Vaccinia virus Accession #: P68698.1Expression system: E. coliBioactivity: One unit of DNA Topoisomerase I from Vaccinia converts 1 µg of supercoiled closed circular pUC18 DNA to relaxed closed circular form in 30 min at 37°C.Synonyms: Vaccinia Virus DNA Topoisomerase I | H6R
