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Gene Editing
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5' DNA/RNA AdenylaseSpecies: Adenosylase gene of thermophilic bacteria Accession #: 8UCH_A,A0A075LQ94Expression system: E. coliBioactivity: One unit is the amount of enzyme required to catalyze the transfer of 1pmol of AMP to pDNA substrate in a total reaction volume of 20μl in 15 minutes at 65℃.Synonyms: Single-Strand Nucleic Acid 5' Adenylase
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AlkB (RNA/DNA demethylase, Nuclease-free)Species: Escherichia coli (strain K12) Accession #: P05050.1Expression system: E. coliBioactivity: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
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Antarctic Phosphatase (Thermosensitive)Species: Antarctic bacterium TAB5 Accession #: 2W5W_B,Q9KWY4Expression system: E. coliBioactivity: 1 unit refers to the 5 'concave end produced by restriction endonuclease digestion at 37 ℃ for 30 minutes. The enzyme amount required for dephosphorylation of µ g pUC19 vector DNA. The dephosphorylation standard refers to the self linking reaction Can inhibit>95% of DNA re circularization and detect it by transforming E. coli cells.Synonyms: Thermosensitive phosphatase
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Klenow Fragment, Exo-Species: E. coli Accession #: 2KFZ_A,P00582Expression system: E. coliBioactivity: One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37℃.Synonyms: DNA polymerase I Fragment Mutant
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Lambda ExonucleaseSynonyms: λ Exonuclease | exo | lambdap24
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SP6 RNA PolymeraseAccession #: Q7Y5N4Expression system: E. coliBioactivity: One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
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T7 Endonuclease IAccession #: P00641.1Expression system: E. coliBioactivity: One unit is defined as the amount of enzyme required to convert > 90% of 1μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37℃.
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Thermolabile dsDNaseAccession #: C9YSL6Expression system: Pichia pastoris
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Cas9 Nuclease (SpCas9)Accession #: Q99ZW2.1Expression system: E. coliSynonyms: CRISPR-associated endonuclease Cas9 | Csn1
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Cas9 NLS (SpCas9-NLS)Accession #: Q99ZW2.1Expression system: E. coliSynonyms: CRISPR-associated endonuclease Cas9 | Csn1 | cas9
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Tn5 TransposaseSpecies: E. coli Accession #: Q46731Expression system: E. coli
