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Gene Editing
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5' DNA/RNA AdenylaseSpecies: Adenosylase gene of thermophilic bacteria Accession #: 8UCH_A,A0A075LQ94Expression system: E. coliBioactivity: One unit is the amount of enzyme required to catalyze the transfer of 1pmol of AMP to pDNA substrate in a total reaction volume of 20μl in 15 minutes at 65℃.Synonyms: Single-Strand Nucleic Acid 5' Adenylase
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AlkB (RNA/DNA demethylase, Nuclease-free)Species: Escherichia coli (strain K12) Accession #: P05050.1Expression system: E. coliBioactivity: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
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Antarctic Phosphatase (Thermosensitive)Species: Antarctic bacterium TAB5 Accession #: 2W5W_B,Q9KWY4Expression system: E. coliBioactivity: 1 unit refers to the 5 'concave end produced by restriction endonuclease digestion at 37 ℃ for 30 minutes. The enzyme amount required for dephosphorylation of µ g pUC19 vector DNA. The dephosphorylation standard refers to the self linking reaction Can inhibit>95% of DNA re circularization and detect it by transforming E. coli cells.Synonyms: Thermosensitive phosphatase
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Klenow Fragment, Exo-Species: E. coli Accession #: 2KFZ_A,P00582Expression system: E. coliBioactivity: One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37℃.Synonyms: DNA polymerase I Fragment Mutant
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Lambda ExonucleaseSynonyms: λ Exonuclease | exo | lambdap24
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SP6 RNA PolymeraseAccession #: Q7Y5N4Expression system: E. coliBioactivity: One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
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T7 Endonuclease IAccession #: P00641.1Expression system: E. coliBioactivity: One unit is defined as the amount of enzyme required to convert > 90% of 1μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37℃.
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Terminal Deoxynucleotidyl Transferase (TdT)Species: Bovine Accession #: P06526Expression system: E. coliBioactivity: One Unit is the amount of enzyme required to catalyze the incorporation of 1 nmol of dNTP to the 3'-OH termini of a polynucleotide within 60 minutes at 37℃.Synonyms: TdT | DNTT | DNA nucleotidylexotransferase | Terminal addition enzyme | Terminal deoxynucleotidyltransferase ...
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Terminal Deoxynucleotidyl Transferase (TdT)Species: Bovine Accession #: P06526Expression system: E. coliBioactivity: One unit is the amount of enzyme required to catalyze the incorporation of 1 nmol of dNTP to the 3'-OH termini of a polynucleotide within 60 minutes at 37℃.Synonyms: TdT | DNTT | DNA nucleotidylexotransferase | Terminal addition enzyme | Terminal deoxynucleotidyltransferase ...
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Endonuclease VIIISpecies: E. coli Accession #: P50465.3Expression system: E. coliBioactivity: One unit is defined as the amount of enzyme required to cleave 1pmol of a 34 mer oligonucleotide duplex containing a single AP site in a total reaction volume of 10μl in 1 hour at 37℃ in 1X Endonuclease VIII Reaction Buffer containing 10pmol of fluorescently labeled oligonucleotide duplexSynonyms: Endonuclease 8 | nei | ECK0703
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Protein A-MNase (pA-MNase)Species: Staphylococcus aureus Accession #: UPI000AAD0772Expression system: E. coliBioactivity: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
