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MHC Class II Rat mAb (PE) - Primary antibody, specific to MHC Class II, Rat IgG2b

COA COA
    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2b
  • Clone Number: M5/114.15.2
  • Conjugation: PE
In stock
Item Number
Ab187212
Grouped product items
SKU Size
Availability
 Price Qty
Ab187212-10μg
10μg
3
$22.90
Ab187212-25μg
25μg
2
$46.90
Ab187212-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$103.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (M5/114.15.2); Rat anti Mouse MHC Class II Antibody (PE); Flow

View related series
Abeta (13) Accession#:O78196 (7) Accession#:P14483 (17) Eb (17) Flow Cytometry (1158) Gene ID:14961 (15) Gene ID:14969 (22) H-2Ab (13) H-2Eb (17) H2-Ab1 (14) H2-Ab1/H2-Eb1 (14) H2Eb (17) I-Abeta (13) Ia-2 (13) Ia-4 (17) Ia2 (13) Ia4 (17) IAb (13) PE (167) Rmcs1 (13)

Basic Description

Product Name MHC Class II Rat mAb (PE) - Primary antibody, specific to MHC Class II, Rat IgG2b
Synonyms E-alpha-f | H-2 class II histocompatibility antigen, E-K alpha chain | H-2 class II histocompatibility antigen, E-U alpha chain | H-2E | H2-Ea | H-2Ea | H2-Ea-ps | I | Ia | Ia3 | Ia-3 | I-E | I-Ealpha | MHC-H2-Ea | MHC class II (I-A/I-E) | MHC class II IA
Specifications & Purity ExactAb™, Validated, Ex:565nm, Em:575nm, 0.5 mg/mL
Host species Rat
Specificity MHC Class II
Immunogen Activated C57BL/6 mouse spleen cells
Positive Control Flow: Mouse splenocytes.
Conjugation PE
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 575nm
Ex/Em(nm) Ex:565nm;Em:575nm
Grade Em:575nm, Ex:565nm, ExactAb™, Validated
Product Description

Flow: ≤0.3 μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Product Properties

Isotype Rat IgG2b
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

MHC Class II Rat mAb (PE) (Ab187212) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with MHC Class II Rat mAb (PE) (Ab187212) at 0.05μg/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (PE) (Ab180312).

MHC Class II Rat mAb (PE) (Ab187212) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with MHC Class II Rat mAb (PE) (Ab187212) at 0.05μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot Number Certificate Type Date Item
ZJ24F0809679 Certificate of Analysis May 23, 2025 Ab187212

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