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MHC Class II Rat mAb (FITC) - Primary antibody, specific to MHC Class II, Rat IgG2b

COA COA
    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2b
  • Clone Number: M5/114.15.2
  • Conjugation: FITC
In stock
Item Number
Ab187211
Grouped product items
SKU Size
Availability
 Price Qty
Ab187211-10μg
10μg
4
$16.90
Ab187211-25μg
25μg
3
$34.90
Ab187211-100μg
100μg
1
$89.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (M5/114.15.2); Rat anti Mouse MHC Class II Antibody (FITC); Flow

View related series
Abeta (13) Accession#:O78196 (7) Accession#:P14483 (17) Eb (17) FITC (181) Flow Cytometry (1158) Gene ID:14961 (15) Gene ID:14969 (22) H-2Ab (13) H-2Eb (17) H2-Ab1 (14) H2-Ab1/H2-Eb1 (14) H2Eb (17) I-Abeta (13) Ia-2 (13) Ia-4 (17) Ia2 (13) Ia4 (17) IAb (13) Rmcs1 (13)

Basic Description

Product Name MHC Class II Rat mAb (FITC) - Primary antibody, specific to MHC Class II, Rat IgG2b
Synonyms E-alpha-f | H-2 class II histocompatibility antigen, E-K alpha chain | H-2 class II histocompatibility antigen, E-U alpha chain | H-2E | H2-Ea | H-2Ea | H2-Ea-ps | I | Ia | Ia3 | Ia-3 | I-E | I-Ealpha | MHC-H2-Ea | MHC class II (I-A/I-E) | MHC class II IA
Specifications & Purity ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL
Host species Rat
Specificity MHC Class II
Immunogen Activated C57BL/6 mouse spleen cells
Positive Control Flow: Mouse splenocytes.
Conjugation FITC
Excitation(nm) Blue Laser(488nm)
Emission(nm) 517nm
Ex/Em(nm) Ex:498nm;Em:517nm
Grade Em:517nm, Ex:498nm, ExactAb™, Validated
Product Description

Flow: ≤0.3 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Product Properties

Isotype Rat IgG2b
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

MHC Class II Rat mAb (FITC) (Ab187211) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with MHC Class II Rat mAb (FITC) (Ab187211) at 0.05μg/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (FITC) (Ab180311). 

MHC Class II Rat mAb (FITC) (Ab187211) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with MHC Class II Rat mAb (FITC) (Ab187211) at 0.05μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (FITC) (Ab180311). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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