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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab190300-10μg
|
10μg |
3
|
$23.90
|
|
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Ab190300-25μg
|
25μg |
3
|
$47.90
|
|
|
Ab190300-100μg
|
100μg |
1
|
$119.90
|
|
mAb (AF6-88.5.5.3); Mouse anti Mouse MHC class I/H-2Kb Antibody (FITC); Flow Cytometry
| Product Name | MHC class I/H-2Kb Mouse mAb (FITC) - Primary antibody, specific to MHC class I/H-2Kb |
|---|---|
| Synonyms | K-f | H-2K | H2-K | H2-D1 | H-2K(d) | A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I |
| Specifications & Purity | ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | MHC class I/H-2Kb |
| Immunogen | C57BL/6 mouse splenocytes |
| Positive Control | Flow: C57BL/6 mouse splenocytes. |
| Conjugation | FITC |
| Excitation(nm) | Blue Laser(488nm) |
| Emission(nm) | 517nm |
| Ex/Em(nm) | Ex:498nm;Em:517nm |
| Grade | Em:517nm, Ex:498nm, ExactAb™, Validated |
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Product Description |
Mouse anti Mouse MHC class I/H-2Kb Antibody, Monoclonal (AF6-88.5.5.3), could be used for in vivo administration, Flow and so on.
|
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
|---|---|
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
MHC class I/H-2Kb Mouse mAb (FITC) (Ab190300) - Flow Cytometry
Flow cytometry analysis of MHC class I/H-2Kb expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with MHC class I/H-2Kb Mouse mAb (FITC) (Ab190300) at 0.2μg/test for 1 hour at 4°C. Left panel - Mouse IgG (FITC) (Ab180307).
MHC class I/H-2Kb Mouse mAb (FITC) (Ab190300) - Flow Cytometry
Flow cytometry analysis of MHC class I/H-2Kb expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with MHC class I/H-2Kb Mouse mAb (FITC) (Ab190300) at 0.2μg/test for 1 hour at 4°C. Blue - Mouse IgG (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry |
≤1.0 μg/test,
the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume
|
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