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MHC class I Mouse mAb (AF647) - Primary antibody, specific to MHC class I

COA COA
    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Mouse
  • Isotype: Mouse IgG2a
  • Clone Number: 34-1-2S
  • Conjugation: AF647
In stock
Item Number
Ab189656
Grouped product items
SKU Size
Availability
 Price Qty
Ab189656-10μg
10μg
3
$49.90
Ab189656-25μg
25μg
2
$89.90
Ab189656-100μg
100μg
1
$219.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (34-1-2S); Mouse anti Mouse MHC class I Antibody (AF647); Flow Cytometry

View related series
Click to view target information: HLA-A
Accession#: P01899 (5) Accession#: P01901 (13) AF647 (119) Flow Cytometry (1158) Gene ID: 14964 (5) Gene ID: 14972 (17) H-2D (4) H-2K (12) H-2K(d) (12) H2-D (4) H2-D1 (13) H2-K (12) H2-K1 (17) K-f (12)

Basic Description

Product Name MHC class I Mouse mAb (AF647) - Primary antibody, specific to MHC class I
Synonyms H-2D | H2-D | H2-K1 | K-f | H-2K | H2-K | H2-D1 | H-2K(d) | A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alph
Specifications & Purity ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL
Host species Mouse
Specificity MHC class I
Immunogen BDF mouse spleen cells
Positive Control Flow: C57BL/6 mouse splenocytes.
Conjugation AF647
Excitation(nm) Red Laser(633nm)
Emission(nm) 668nm
Ex/Em(nm) Ex:650nm;Em:668nm
Grade Em:668nm, Ex:650nm, ExactAb™, Validated
Product Description

Mouse anti Mouse MHC class I Antibody, Monoclonal (34-1-2S), could be used for Flow, in vivo activation of APCs and so on.

Product Properties

Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

HLA-A Tbio HLA class I histocompatibility antigen, A-31 alpha chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

MHC class I Mouse mAb (AF647) (Ab189656) - Flow Cytometry
Flow cytometry analysis of MHC class I expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with MHC class I Mouse mAb (AF647) (Ab189656) at 0.2μg/test for 1 hour at 4°C. Left panel - Mouse IgG (AF647) (Ab190077). 

MHC class I Mouse mAb (AF647) (Ab189656) - Flow Cytometry
Flow cytometry analysis of MHC class I expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with MHC class I Mouse mAb (AF647) (Ab189656) at 0.2μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF647) (Ab190077). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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