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Gemtuzumab (anti-CD33) - Primary antibody, specific to CD33, >95%, high purity, Human IgG4SP, BINDING AGENT of Myeloid cell surface antigen CD33 binding agent, BINDING AGENT of Myeloid cell surface antigen CD33 binding agent

    Application:
  • Animal Model
  • ELISA
  • Flow Cytometry
  • Functional Assay
  • Host species: Human
  • Reactive species: Human
  • Isotype: Human IgG4SP
  • Conjugation: Unconjugated
In stock
Item Number
Ab175859
Grouped product items
SKU Size
Availability
Price Qty
Ab175859-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab175859-1mg
1mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$327.90
Ab175859-5mg
5mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$807.90
Ab175859-10mg
10mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,287.90

Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG4SP; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

Basic Description

Product Name Gemtuzumab (anti-CD33) - Primary antibody, specific to CD33, >95%, high purity, Human IgG4SP, BINDING AGENT of Myeloid cell surface antigen CD33 binding agent
Synonyms CD 33 antibody | CD33 antibody | CD33 antigen (gp67) antibody | CD33 antigen antibody | CD33 molecule antibody | CD33_HUMAN antibody | FLJ00391 antibody | gp67 antibody | My9 antibody | Myeloid cell surface antigen CD33 antibody | Myeloid cell surface ant
Specifications & Purity Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA
Host species Human
Specificity CD33
Conjugation Unconjugated
Grade Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated
Action Type BINDING AGENT
Mechanism of action BINDING AGENT of Myeloid cell surface antigen CD33 binding agent
Product Description

Gemtuzumab (anti-CD33) is a monoclonal antibody targeting CD33 antigen, which present on leukemic myeloblasts of acute myeloid leukemia (AML). Gemtuzumab (anti-CD33) can be used for synthesis of antibody-drug conjugate (ADC), Gemtuzumab ozogamicin. Gemtuzumab ozogamicin consists of a cytotoxic derivative of Calicheamicin (a cytotoxic antibiotic), and a monoclonal antibody. Gemtuzumab ozogamicin can be used for the research of acute myeloid leukemia.
Purity
>95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Product Properties

Isotype Human IgG4SP
Light Chain Type kappa
SDS-PAGE 27.4 kDa (Light Chain) & 53.5 kDa (Heavy Chain), under reducing conditions; 175.3 kDa, under non-reducing conditions.
Purification Method Protein A purified
Purity >95%
Shape Liquid
Concentration See COA
Storage Temp Store at -80°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
CAS 220578-59-6

Associated Targets(Human)

CD33 Tclin Myeloid cell surface antigen CD33 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Gemtuzumab (anti-CD33) (Ab175859) - Flow Cytometry
Flow Cytometry analysis of THP-1 cells labelling CD33 (red) with Gemtuzumab (anti-CD33) (Ab175859). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG4SP. Black - Unlabelled control, cells without incubation with primary antibody.

Gemtuzumab (anti-CD33) (Ab175859) - SEC
The purity of Gemtuzumab (anti-CD33) (Ab175859) is more than 95% verified by HPLC.

Gemtuzumab (anti-CD33) (Ab175859) - ELISA
Immobilized Recombinant Human CD33 Protein (rp143966) at 2.0 μg/mL can bind Gemtuzumab (anti-CD33) (Ab175859). The ED₅₀ for this effect is 391.3 ng/mL.

Gemtuzumab (anti-CD33) (Ab175859) - ELISA
Immobilized Recombinant Human Siglec-3/CD33 Protein (rp169578) at 1.0 μg/mL can bind Gemtuzumab (anti-CD33) (Ab175859) with the EC₅₀ of 36.93 ng/mL.


Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0900969 Certificate of Analysis Sep 06, 2023 Ab175859
ZJ23F0900970 Certificate of Analysis Sep 06, 2023 Ab175859

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