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Nucleases (DNases and RNases)
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Recombinant Taq DNA Polymerase ProteinCas Number: 9012-90-2Formula: Molecular Weight: 94 kDaAccession #: P19821Expression system: E. coli Protein Tag: N-HisBioactivity: Taq DNA polymerase is a heat-resistant enzyme that synthesizes DNA from a single-stranded template in the presence of dNTP and primers, with 5´→3´ DNA polymerase activity and 5´→3´ exonuclease activity.Synonyms: DNA polymerase I | thermostable | DNA-directed DNA polymerase
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Protein A-MNase (pA-MNase)Species: Staphylococcus aureus Accession #: UPI000AAD0772Expression system: E. coliBioactivity: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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Protein A/G-MNase (pAG-MNase)Species: Staphylococcus aureus Accession #: UPI000AAD0772Expression system: E. coliBioactivity: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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Protein G-MNase (pG-MNase)Species: Staphylococcus aureus Accession #: UPI000AAD0772Expression system: E. coliBioactivity: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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RNase HIISpecies: Escherichia coli (strain K12) Accession #: P10442Expression system: E. coliBioactivity: One unit refers to the amount of enzyme required to cleave 1 nmol per minute of a synthetic DNA/RNA hybrid double stranded substrate containing a single rC at 37 ° CSynonyms: Ribonuclease HII | RNase H2 | rnhB | ECK0182 | Recombinant RNase HII
