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Microbiology and Virology
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Recombinant Human SARS-CoV-2 Spike Protein RBDSpecies: SARS-CoV-2 Accession #: P0DTC2Expression system: HEK293 Protein Tag: C-His Endotoxin Concentration: <1.0 EU/μgBioactivity: 1. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 5.0 μg/mL can bind Recombinant Human ACE-2 Protein (rp176242) with the EC50 of 75.79 ng/mL. 2. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Regdanvimab (anti-Spike RBD) (Ab182925) with the EC50 of 12.65 ng/mL. 3. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Imdevimab (anti-Spike RBD) (Ab182897) with the EC50 of 7.82 ng/mL. 4. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Etesevimab (anti-Spike RBD) (Ab182932) with the EC50 of 23.96 ng/mL. 5. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Sotrovimab (anti-Spike RBD) (Ab182938) with the EC50 of 41.33 ng/mL. 6. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Casirivimab (anti-Spike RBD) (Ab182896) with the EC50 of 10.50 ng/mL.Synonyms: SARS-CoV-2 RBD Protein | SARS-CoV-2 Spike RBD Protein | SARS-CoV-2 S Protein RBD
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Recombinant EN-TEV Protease ProteinSpecies: Tobacco etch virus(TEV) Accession #: P04517Expression system: E. coli Protein Tag: N-His Endotoxin Concentration: <1.0 EU/μgBioactivity: Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser , with the cleavage point after Gln. One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.Complete digestion of 50µg of fusion KGF-MBP tag protein using 1µg TEV enzyme (in 1/50 ratio) in either 1 hours at 30 °C or overnight at 4 °C. The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT.Synonyms: NIa | P1 Protease | TEV Protease | Tobacco Etch Virus Protease
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T4 DNA PolymeraseSpecies: Escherichia phage T4 Accession #: P04415Expression system: E. coliBioactivity: Catalytic incorporation of 10nmol deoxyribonucleotides (dNTPs) into polynucleotides at 37 ℃ for 30 minutes. The required enzyme quantity is defined as one active unit.Synonyms: T4DNAPolymerase | Eta2 | DNA-directed DNA polymerase
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Recombinant Mouse ACE-2 ProteinSpecies: Mouse Accession #: Q8R0I0Expression system: HEK293 Protein Tag: C-10His Endotoxin Concentration: <0.1 EU/μgBioactivity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The specific activity is >400 pmol/min/µg, as measured under the described conditions.Synonyms: ACE-2 | ACE2 | ACEH | ACEHangiotensin I converting enzyme 2 | ACE-related carboxypeptidase | angiotensin I converting...
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Recombinant Human TEM8/ANTXR1 ProteinSpecies: Human Accession #: Q9H6X2Expression system: HEK293 Protein Tag: C-His Endotoxin Concentration: <0.1 EU/μgBioactivity: Measured by its binding ability in a functional ELISA. When Anthrax Protective Antigen is coated at 1.5 μg/mL (100 μL/well), the concentration of Recombinant Human TEM8/ANTXR1 that produces 50% optimal binding response is 0.6-3.6 μg/mL.Synonyms: ANTXR1 | anthrax toxin receptor 1 | ATR2310008J16Rik | FLJ10601 | FLJ11298 | FLJ21776 | TEM8 | TEM82810405N18Rik | Tu...
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Recombinant Human PGLYRP1/PGRP-S ProteinSpecies: Human Accession #: O75594Expression system: HEK293 Protein Tag: C-His Endotoxin Concentration: <0.1 EU/μgBioactivity: Measured by its binding ability in a functional ELISA. When peptidoglycan is coated at 1 µg/mL (100 µL/well), the concentration of Recombinant Human PGLYRP1/PGRP-S that produces 50% optimal binding response is 0.75-4.5 ng/mL.Synonyms: Peptidoglycan recognition protein short | PGRP-S | peptidoglycan recognition protein 1 | Peptidoglycan recognition pr...
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5' DNA/RNA AdenylaseSpecies: Adenosylase gene of thermophilic bacteria Accession #: 8UCH_A,A0A075LQ94Expression system: E. coliBioactivity: One unit is the amount of enzyme required to catalyze the transfer of 1pmol of AMP to pDNA substrate in a total reaction volume of 20μl in 15 minutes at 65℃.Synonyms: Single-Strand Nucleic Acid 5' Adenylase
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AlkB (RNA/DNA demethylase, Nuclease-free)Species: Escherichia coli (strain K12) Accession #: P05050.1Expression system: E. coliBioactivity: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
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Antarctic Phosphatase (Thermosensitive)Species: Antarctic bacterium TAB5 Accession #: 2W5W_B,Q9KWY4Expression system: E. coliBioactivity: 1 unit refers to the 5 'concave end produced by restriction endonuclease digestion at 37 ℃ for 30 minutes. The enzyme amount required for dephosphorylation of µ g pUC19 vector DNA. The dephosphorylation standard refers to the self linking reaction Can inhibit>95% of DNA re circularization and detect it by transforming E. coli cells.Synonyms: Thermosensitive phosphatase
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Bst DNA Polymerase, Large FragmentSpecies: Geobacillus stearothermophilus Accession #: Q45458Expression system: E. coliBioactivity: One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTP into acid insoluble material in 30 minutes at 65℃.Synonyms: (Bacillus stearothermophilus, Bst) DNA Polymerase I , Large Fragment | DNA polymerase I | polA
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Cre RecombinaseSpecies: Escherichia phage P1 Accession #: Q5QBM7Expression system: E. coliBioactivity: One unit is defined as the amount of enzyme required to achieve the maximum site-specific recombination of 250ng of control DNA in 30 minutes at 37ºC in a 50μl reaction system. The maximum recombination can be analyzed by agarose gel electrophoresis or by screening on the corresponding resistance plates after transformation of the reactions.Synonyms: Cyclization Recombination Enzyme | Type I topoisomerase | tyrosine recombinase
