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HO-1 Mouse mAb - Primary antibody, specific to HO-1, IgG2a

COA COA
    Application:
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2a
  • Clone Number: CD02/4E2
  • Conjugation: Unconjugated
In stock
Item Number
Ab181869
Grouped product items
SKU Size
Availability
 Price Qty
Ab181869-10μl
10μl
≥10
$99.90
Ab181869-50μl
50μl
8
$189.90
Ab181869-100μl
100μl
4
$299.90
Ab181869-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (CD02/4E2); Mouse anti Human HO-1 Antibody; WB, ELISA, ICC/IF; Unconjugated

View related series
Click to view target information: HMOX1
Accession#:P09601 (5) Gene ID:3162 (5) HMOX1 (6) HO-1 (3)

Basic Description

Product Name HO-1 Mouse mAb - Primary antibody, specific to HO-1, IgG2a
Synonyms 32 kD antibody | bK286B10 antibody | D8Wsu38e antibody | heat shock protein 32 kD antibody | heat shock protein 32kD antibody | Heat shock protein antibody | Heme oxygenase (decycling) 1 antibody | Heme oxygenase 1 antibody | Hemox antibody | HMOX 1 antib
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
Host species Mouse
Specificity HO-1
Immunogen Recombinant protein corresponding to Human HO-1
Positive Control WB: HeLa, HEK293, HepG2 and A549 whole cell lysates ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application

WB: 1/1,000 - 1/200,000; Predicted molecular weight: 32.8 KDa

ICC/IF: 1/200

Product Properties

Isotype IgG2a
Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

HMOX1 Tchem Heme oxygenase 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

HO-1 Mouse mAb (Ab181869) - Western Blot
All lanes: HO-1 Mouse Antibody (Ab181869) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 30 kDa
Exposure time: 8.7 s 

HO-1 Mouse mAb (Ab181869) - ICC/IF
IF analysis of HO-1(Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with HO-1 Mouse mAb (Ab181869) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
WB

1/1,000 - 1/200,000; Predicted molecular weight: 32.8 KDa

IF/ICC

1/200

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0404925 Certificate of Analysis Apr 26, 2024 Ab181869
ZJ24F0404924 Certificate of Analysis Apr 26, 2024 Ab181869
ZJ24F0404923 Certificate of Analysis Apr 26, 2024 Ab181869

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