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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab191420-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$89.90
|
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Ab191420-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$269.90
|
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Ab191420-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$379.90
|
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Ab191420-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,899.90
|
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mAb (K92016_6B11); Mouse anti Human GBP6 Antibody; WB, IHC; Unconjugated
| Product Name | GBP6 Mouse mAb - Primary antibody, specific to GBP6 |
|---|---|
| Synonyms | GBP-6 | GTP-binding protein 6 | Guanine nucleotide-binding protein 6 | guanylate binding protein family, member 6 | Guanylate-binding protein 6 |
| Specifications & Purity | ExactAb™, Carrier Free, Validated, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens, such as bacterial pathogens Listeria monocytogenes and Mycobacterium bovis BCG as well as the protozoan pa |
| Host species | Mouse |
| Specificity | GBP6 |
| Immunogen | Recombinant protein of human GBP6 (AA 291-570) |
| Positive Control | WB: Mouse liver, A431 lysates. IHC: Human esophagus tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human GBP6 Antibody, Monoclonal (K92016_6B11), could be used for WB, IHC and so on.
|
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
GBP6 Mouse mAb (Ab191420) - Western Blot
All lanes: GBP6 Mouse mAb (Ab191420) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 69 kDa
Exposure time: 70.5 s
GBP6 Mouse mAb (Ab191420) - Western Blot
All lanes: GBP6 Mouse mAb (Ab191420) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 69 kDa
Exposure time: 60.0 s
GBP6 Mouse mAb (Ab191420) - IHC
IHC analysis of esophagus tissue by GBP6 Mouse mAb (Ab191420).
IHC was performed using sections of the formalin-fixed paraffin-embedded esophagus tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with GBP6 Mouse mAb (Ab191420) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Squamous epithelial cells are positively stained at the cytoplasm.
| Application | Dilution info |
|---|---|
| WB |
1/1000
|
| IHC | 1/100 |