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ECT2 Mouse mAb - Primary antibody, specific to ECT2, Mouse IgG1

    Application:
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse
  • Isotype: Mouse IgG1
  • Clone Number: 1691CT516.3.3
  • Conjugation: Unconjugated
In stock
Item Number
Ab100925
Grouped product items
SKU Size
Availability
Price Qty
Ab100925-10μl
10μl
2
$69.90
Ab100925-50μl
50μl
1
$199.90
Ab100925-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab100925-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,799.90

mAb (1691CT516.3.3); Mouse anti Human ECT2 Antibody; WB; Unconjugated

Basic Description

Product Name ECT2 Mouse mAb - Primary antibody, specific to ECT2, Mouse IgG1
Synonyms ARHGEF31 | ECT 2 antibody | ECT2 antibody | ECT2 protein antibody | ECT2_HUMAN antibody | Epithelial cell transforming sequence 2 antibody | Epithelial cell transforming sequence 2 oncogene antibody | Epithelial cell transforming sequence 2 oncogene prote
Specifications & Purity ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Host species Mouse
Specificity ECT2
Immunogen Recombinant ECT2 expressed in E.coli (AA 200-460)
Positive Control WB: HT-29, A549 and SK-OV-3 cell lysates.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human ECT2 Antibody, Monoclonal (1691CT516.3.3), could be used for WB and so on.

Application

WB: 1/1000

Protein Function

Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ECT2 Tbio Protein ECT2 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

ECT2 Mouse mAb (Ab100925) - Western Blot
All lanes: ECT2 Mouse mAb (Ab100925) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 104 kDa
Observed band size: 104 kDa
Exposure time: 254.0 s

ECT2 Mouse mAb (Ab100925) - Western Blot
All lanes: ECT2 Mouse mAb (Ab100925) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 104 kDa
Observed band size: 104 kDa
Exposure time: 0.5 s

Application

Application Dilution info
WB

1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0303280 Certificate of Analysis Mar 14, 2024 Ab100925
ZJ24F0303279 Certificate of Analysis Mar 14, 2024 Ab100925

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