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Density Regulated Protein Mouse mAb - Primary antibody, specific to Density Regulated Protein, Mouse IgG2b

    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2b
  • Clone Number: F42S106
  • Conjugation: Unconjugated
In stock
Item Number
Ab099620
Grouped product items
SKU Size
Availability
Price Qty
Ab099620-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab099620-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$149.90
Ab099620-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$249.90
Ab099620-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (F42S106); Mouse anti Human Density Regulated Protein Antibody; WB, IHC, IF/ICC, Flow Cytometry; Unconjugated

Basic Description

Product Name Density Regulated Protein Mouse mAb - Primary antibody, specific to Density Regulated Protein, Mouse IgG2b
Synonyms denr antibody | DENR_HUMAN antibody | Density regulated protein antibody | Density-regulated protein antibody | DRP antibody | DRP1 antibody | DRP1 protein antibody | Protein DRP1 antibody | SMAP-3 antibody | SMAP3 antibody | Smooth muscle cell associated
Specifications & Purity ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Biochemical and Physiological Mechanisms Translation regulator forming a complex with MCTS1 to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream OR
Host species Mouse
Specificity Density Regulated Protein
Immunogen Recombinant protein of human Density Regulated Protein.
Positive Control WB: A431, HeLa, HEK293 and Jurkat cell lysates. IHC: Human skeletal muscle tissue. IF/ICC: HeLa cells. Flow Cytometry: HeLa cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human Density Regulated Protein Antibody, Monoclonal (F42S106), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Product Properties

Isotype Mouse IgG2b
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

DENR Tbio Density-regulated protein (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Density Regulated Protein Mouse mAb (Ab099620) - Western Blot
All lanes: Density Regulated Protein Mouse mAb (Ab099620) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 29 kDa
Exposure time: 9.7 s

Density Regulated Protein Mouse mAb (Ab099620) - Western Blot
All lanes: Density Regulated Protein Mouse mAb (Ab099620) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 29 kDa
Exposure time: 9.7 s

Density Regulated Protein Mouse mAb (Ab099620) - IHC
Immunohistochemical analysis of DENR staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Density Regulated Protein Mouse mAb (Ab099620) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Density Regulated Protein Mouse mAb (Ab099620) - IF/ICC
Immunofluorescent analysis of DENR staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Density Regulated Protein Mouse mAb (Ab099620) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Density Regulated Protein Mouse mAb (Ab099620) - Flow Cytometry
Flow cytometric analysis of HeLa cells using Density Regulated Protein Mouse mAb (Ab099620). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Density Regulated Protein Mouse mAb (Ab099620) at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Application

Application Dilution info
Flow Cytometry

1/10-1/50

IHC

1/50-1/200

IF/ICC

1/10 - 1/50

WB

1/500-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

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