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Chk1 Mouse mAb - Primary antibody, specific to Chek1, IgG1

    Application:
  • ELISA
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse
  • Isotype: Mouse IgG1
  • Clone Number: 2G1D5
  • Conjugation: Unconjugated
In stock
Item Number
Ab096310
Grouped product items
SKU Size
Availability
Price Qty
Ab096310-10μl
10μl
4
$89.90
Ab096310-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$267.90
Ab096310-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$327.90
Ab096310-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,177.90

mAb(2G1D5); Mouse anti Human Chk1 Antibody; WB, ELISA; Unconjugated

Basic Description

Product Name Chk1 Mouse mAb - Primary antibody, specific to Chek1, IgG1
Synonyms C85740 antibody | Cell cycle checkpoint kinase antibody | Checkpoint , S. pombe, homolog of, 1 antibody | Checkpoint kinase 1 antibody | Checkpoint kinase 1 homolog (S. pombe) antibody | CHEK 1 antibody | Chek1 antibody | Chk 1 antibody | Chk1 antibody |
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species Mouse
Specificity Chek1
Immunogen Purified recombinant fragment of CHK1 (AA: 207-421) expressed in E.coli
Positive Control WB: A431, Hela, NIH/3T3 and K562 cell lysate. HEK293T cells transfected with pCMV6-ENTRY CHK1 cDNA.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse Monoclonal (2G1D5) to CHK1, could be used for WB and ELISA.
Application:
WB: 1/500 - 1/2000
ELISA: 1/10000

Protein Function:
CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Product Properties

Isotype IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C. Avoid freeze/thaw cycle.

Associated Targets(Human)

CHEK1 Tchem Serine/threonine-protein kinase Chk1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Chk1 Mouse mAb (Ab096310) - Western Blot
All lanes: Chk1 Mouse mAb (Ab096310) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Chk1 Mouse mAb (Ab096310) - Western Blot
All lanes: Chk1 Mouse mAb (Ab096310) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Application

Application Dilution info
WB

1/500-1/2000

ELISA

1/10000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0303042 Certificate of Analysis Mar 08, 2024 Ab096310
ZJ23F0700740 Certificate of Analysis Aug 01, 2023 Ab096310
ZJ23F0700741 Certificate of Analysis Aug 01, 2023 Ab096310

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