Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab095606-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
|
|
|
Ab095606-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$209.90
|
|
|
Ab095606-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
|
|
Ab095606-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
|
|
mAb (F35S627); Mouse anti Human CDC25C Antibody; WB, IHC; Unconjugated
| Product Name | CDC25C Mouse mAb - Primary antibody, specific to CDC25C, Mouse IgG2a |
|---|---|
| Synonyms | CDC 25 antibody | Cdc 25C antibody | CDC25 antibody | CDC25C antibody | Cell division cycle 25 homolog C antibody | Cell division cycle 25C antibody | Cell division cycle 25C protein antibody | Dual specificity phosphatase Cdc25C antibody | M phase induce |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity. Post-translational: Phosphorylated by CHK1 on |
| Host species | Mouse |
| Specificity | CDC25C |
| Immunogen | Recombinant fusion protein of human CDC25C. |
| Positive Control | WB: Hep G2, K562, MCF7, Raji and HEK293 cell lysates. IHC: Human testis tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human CDC25C Antibody, Monoclonal (F35S627), could be used for WB, IHC and so on. |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
CDC25C Mouse mAb (Ab095606) - Western Blot
All lanes: CDC25C Mouse mAb (Ab095606) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 55 kDa
Exposure time: 12.6 s
CDC25C Mouse mAb (Ab095606) - IHC
Immunohistochemical analysis of CDC25C staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with CDC25C Mouse mAb (Ab095606) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
| Application | Dilution info |
|---|---|
| IHC | 1/50-1/200 |
| WB |
1/500-1/1000
|