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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab190484-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$19.90
|
|
|
Ab190484-25μg
|
25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$39.90
|
|
|
Ab190484-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$99.90
|
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mAb (OKT-8); Mouse anti Human CD8 alpha Antibody (Biotin); Flow Cytometry
| Product Name | CD8 alpha Mouse mAb (Biotin) - Primary antibody, specific to CD8 alpha |
|---|---|
| Synonyms | CD8 | Leu2 | IMD116 | CD8alpha | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | Biotin Anti-Human CD8a Antibody[OKT-8] | Biotin Anti-Human CD8a (OKT8) | Biotin Mouse Anti-Human CD8 | CD8a Monoclonal Antibody (OKT-8), Biotin | Biotin anti-human |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
| Host species | Mouse |
| Specificity | CD8 alpha |
| Immunogen | Human CD8α |
| Positive Control | Flow: Human peripheral blood lymphocytes |
| Conjugation | Biotin |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti Human CD8 alpha Antibody, Monoclonal (OKT-8), could be used for Flow, in vivo CD8+ T cell depletion in humanized mice and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
CD8 alpha Mouse mAb (Biotin) (Ab190484) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Mouse mAb (Biotin) (Ab190484) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).
CD8 alpha Mouse mAb (Biotin) (Ab190484) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Mouse mAb (Biotin) (Ab190484) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry |
≤0.3 μg/test,
the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume
|
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