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CD8 alpha Mouse mAb (Biotin) - Primary antibody, specific to CD8 alpha

    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2a
  • Clone Number: OKT-8
  • Conjugation: Biotin
In stock
Item Number
Ab190484
Grouped product items
SKU Size
Availability
Price Qty
Ab190484-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$19.90
Ab190484-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$39.90
Ab190484-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90

mAb (OKT-8); Mouse anti Human CD8 alpha Antibody (Biotin); Flow Cytometry

Basic Description

Product Name CD8 alpha Mouse mAb (Biotin) - Primary antibody, specific to CD8 alpha
Synonyms CD8 | Leu2 | IMD116 | CD8alpha | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | Biotin Anti-Human CD8a Antibody[OKT-8] | Biotin Anti-Human CD8a (OKT8) | Biotin Mouse Anti-Human CD8 | CD8a Monoclonal Antibody (OKT-8), Biotin | Biotin anti-human
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Host species Mouse
Specificity CD8 alpha
Immunogen Human CD8α
Positive Control Flow: Human peripheral blood lymphocytes
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Mouse anti Human CD8 alpha Antibody, Monoclonal (OKT-8), could be used for Flow, in vivo CD8+ T cell depletion in humanized mice and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD8 alpha Mouse mAb (Biotin) (Ab190484) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Mouse mAb (Biotin) (Ab190484) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

CD8 alpha Mouse mAb (Biotin) (Ab190484) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Mouse mAb (Biotin) (Ab190484) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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