Determine the necessary mass, volume, or concentration for preparing a solution.
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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab185380-10T
|
10T |
10
|
$49.90
|
|
|
Ab185380-25T
|
25T |
10
|
$95.90
|
|
|
Ab185380-100T
|
100T |
2
|
$209.90
|
|
mAb (CD28.2); Mouse anti Human CD28 Antibody (PE); Flow
| Product Name | CD28 Mouse mAb (PE) - Primary antibody, specific to CD28, Mouse IgG1 |
|---|---|
| Synonyms | CD28 antigen (Tp44) | CD28 antigen | CD28 molecule | CD28 | MGC138290 | T-cell-specific surface glycoprotein CD28 | Tp44 | CD 28 antibody | CD28 antibody | CD28 antigen antibody | CD28 molecule antibody | CD28_HUMAN antibody | MGC138290 antibody | T cell |
| Specifications & Purity | ExactAb™, Validated, Ex:565nm, Em:575nm, 5 μL/test |
| Host species | Mouse |
| Specificity | CD28 |
| Immunogen | Human CD28 transfected cell line |
| Positive Control | Flow: Jurkat |
| Conjugation | PE |
| Excitation(nm) | Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm) |
| Emission(nm) | 575nm |
| Ex/Em(nm) | Ex:565nm;Em:575nm |
| Grade | Em:575nm, Ex:565nm, ExactAb™, Validated |
| Product Description |
Application: |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| Purification Method | Protein G purified |
| Concentration | 5 μL/test |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
CD28 Mouse mAb (PE) (Ab185380) - Flow Cytometry
Flow cytometry analysis of CD28 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD28 Mouse mAb (PE) (Ab185380) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | 5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Jun 18, 2024 | Ab185380 | |
| Certificate of Analysis | Jun 18, 2024 | Ab185380 | |
| Certificate of Analysis | Jun 18, 2024 | Ab185380 | |
| Certificate of Analysis | May 31, 2024 | Ab185380 |
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