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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab211655-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$49.90
|
|
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Ab211655-25μg
|
25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$89.90
|
|
|
Ab211655-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$203.90
|
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mAb (PC-61.5.3); Rat anti Mouse CD25 Antibody (AF405); Flow Cytometry
| Product Name | CD25 Rat mAb (AF405) - Primary antibody, specific to CD25 |
|---|---|
| Synonyms | Anti-mouse CD25 (IL-2Rα)-InVivo | In Vivo MAb anti-mouse CD25 (IL-2Rα) | CD25 Rat mAb | CD25 | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2 receptor sub |
| Specifications & Purity | ExactAb™, Validated, Ex:401nm, Em:422nm, 0.5 mg/mL |
| Host species | Rat |
| Specificity | CD25 |
| Immunogen | IL-2-dependent cytolytic mouse T cell clone B6.1 |
| Positive Control | Flow: C57BL/6 mouse splenocytes |
| Conjugation | AF405 |
| Excitation(nm) | Violet Laser(405nm) |
| Emission(nm) | 422nm |
| Ex/Em(nm) | Ex:401nm;Em:422nm |
| Grade | Em:422nm, Ex:401nm, ExactAb™, Validated |
| Product Description |
Rat anti Mouse CD25 Antibody, Monoclonal (PC-61.5.3), could be used for Flow Cytometry
|
| Light Chain Type | lambda |
|---|---|
| SDS-PAGE | 150kDa |
| Purification Method | Protein A purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
CD25 Rat mAb (AF405) (Ab211655) - Flow Cytometry
Con A-stimulated (2 days) C57BL/6 mouse splenocytes (right panel) stained with CD25 Rat mAb (AF405) (Ab211655) (red) compared to an isotype control (blue). Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. |
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