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CD25 Rat mAb (Biotin) - Primary antibody, specific to CD25, IgG1

COA COA
    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG1
  • Clone Number: PC-61.5.3
  • Conjugation: Biotin
In stock
Item Number
Ab179014
Grouped product items
SKU Size
Availability
 Price Qty
Ab179014-10μg
10μg
10
$29.90
Ab179014-25μg
25μg
3
$49.90
Ab179014-100μg
100μg
2
$89.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb(PC-61.5.3); Rat anti Mouse CD25 Antibody (Biotin); Flow

View related series
Click to view target information: IL2RA
Accession#:P01590 (5) CD25 (12) Flow Cytometry (1158) Gene ID:16184 (6) Il2r (4) IL2RA (14) Ly-43 (4)

Basic Description

Product Name CD25 Rat mAb (Biotin) - Primary antibody, specific to CD25, IgG1
Synonyms Anti-mouse CD25 (IL-2Rα)-InVivo | In Vivo MAb anti-mouse CD25 (IL-2Rα) | CD25 Rat mAb | CD25, Il2r, Ly-43 | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2
Specifications & Purity ExactAb™, Validated, Azide Free, 1.0 mg/mL
Host species Rat
Specificity CD25
Immunogen IL-2-dependent cytolytic mouse T cell clone B6.1
Positive Control Flow: Murine splenocytes
Conjugation Biotin
Grade Azide Free, ExactAb™, Validated
Product Description

Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Product Properties

Isotype IgG1
Light Chain Type lambda
Purification Method Protein A High Salt
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
IL2RA Tclin Interleukin-2 receptor alpha chain (2 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD25 Rat mAb (Biotin) (Ab179014) - Flow Cytometry
BALB/c mouse splenocytes are stained with CD25 Rat mAb (Biotin) (Ab179014) and CD4 Rat mAb (APC) (Ab181112) (right), followed by Recombinant Streptavidin Protein (R-PE) (rp156150). BALB/c mouse splenocytes are stained with CD4 Rat mAb (APC) (Ab181112) and Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left).

CD25 Rat mAb (Biotin) (Ab179014) - Flow Cytometry
Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (Biotin) (Ab179014) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).

Application

Application Dilution info
Flow Cytometry

≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0404894 Certificate of Analysis Jan 16, 2025 Ab179014
ZJ24F0404895 Certificate of Analysis Jan 16, 2025 Ab179014
ZJ24F0404896 Certificate of Analysis Apr 25, 2024 Ab179014

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