Articles du produit groupé

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Taille

Disponibilité

Prix

Qté

Ab156188-10μl

10μl

Available within 8-12 weeks(?)

66,90$US

Ab156188-50μl

50μl

199,90$US

Ab156188-100μl

100μl

299,90$US

Ab156188-1ml

1ml

Available within 8-12 weeks(?)

1 499,90$US

Description générale

Product Name	Vimentin Mouse mAb - Primary antibody, specific to Vimentin, IgG2a
Synonymes	CTRCT30 antibody \| Epididymis luminal protein 113 antibody \| FLJ36605 antibody \| HEL113 antibody \| VIM antibody \| VIME_HUMAN antibody \| Vimentin antibody \| Anti-Vimentin Antibody \| Vimentin Monoclonal Antibody \| Anti-Human VIM/Vimentin Antibody \| Mouse an
Spécifications et pureté	ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
Espèces hôtes	Mouse
Spécificité	Vimentin
Immunogène	Recombinant full length protein corresponding to human Vimentin
Contrôle positif	WB: HeLa, HEK293, A549, Jurkat and NIH/3T3 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells
Conjugaison	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description	Application WB: 1/2,000-1/20,000 ; Predicted molecular weight: 54 kDa Flow: 0.016 μg/mL – 2 μg/mL ICC/IF: 2 μg/mL

Propriétés du produit

Isotype	IgG2a
Type de chaîne lumineuse	kappa
SDS-PAGE	150 kDa
Méthode de purification	Protein A purified
Forme	Liquid
Concentration	1.0 mg/mL
Température de stockage	Store at -20°C,Avoid repeated freezing and thawing
Expédié en	Ice chest + Ice pads
Stabilité et stockage	Store at 4°C for short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Cibles associées (humaines)

VIM Tbio Vimentin (0 Activities)

Discover Target: VIM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	Références

Images

Western Blot: Vimentin Mouse mAb (Ab156188)

Vimentin Mouse mAb (Ab156188) - Western Blot
All lanes: Vimentin Antibody (Ab156188) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 53.7 kDa
Observed band size: 54.9 kDa
Exposure time: 26.9 s

Flow Cytometry: Vimentin Mouse mAb (Ab156188)

Vimentin Mouse mAb (Ab156188) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab156188) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.

ICC/IF: Vimentin Mouse mAb (Ab156188)

Vimentin Mouse mAb (Ab156188) - ICC/IF
IF analysis of Vimentin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vimentin Mouse mAb (Ab156188) at 2 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application	Dilution info
Flow Cytometry	0.016-2.0μg/mL
IF/ICC	2 μg/mL
WB	1/2,000-1/20,000 ; Predicted molecular weight: 54 kDa

Certificats (CoA, COO, BSE/TSE et tableau d'analyse)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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2 results found

Lot Number	Certificate Type	Date	Article
ZJ23F1101670	Certificate of Analysis	Nov 16, 2023	Ab156188
ZJ23F1101669	Certificate of Analysis	Nov 16, 2023	Ab156188

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FAQ et articles

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: Protocols

Tags:

Frozen section Immunofluorescence staining Tissue section Methanol permeability IF

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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Vimentin Mouse mAb - Primary antibody, specific to Vimentin, IgG2a

Description générale

Propriétés du produit

Cibles associées (humaines)

Mécanismes d'action

Images

Application

Certificats (CoA, COO, BSE/TSE et tableau d'analyse)

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Avis des clients

SKU	Taille	Disponibilité	Prix
Ab156188-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	66,90$US
Ab156188-50μl	50μl	8	199,90$US
Ab156188-100μl	100μl	7	299,90$US
Ab156188-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	1 499,90$US

Vimentin Mouse mAb - Primary antibody, specific to Vimentin, IgG2a

Description générale

Propriétés du produit

Cibles associées (humaines)

Mécanismes d'action

Images

Application

Certificats (CoA, COO, BSE/TSE et tableau d'analyse)

Produits apparentés

Nous avons trouvé d’autres produits qui pourraient vous intéresser !

FAQ et articles

Calculateurs de solution

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

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