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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab100725-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
|
|
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Ab100725-50μl
|
50μl |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$207.90
|
|
|
Ab100725-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$329.90
|
|
|
Ab100725-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$3,237.90
|
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mAb (7H12); Mouse anti Human E Cadherin Antibody; WB, IHC, Flow, ELISA; Unconjugated
| Product Name | E Cadherin Mouse mAb - Primary antibody, specific to E Cadherin, IgG1 |
|---|---|
| Synonyms | Arc 1 antibody | CADH1_HUMAN antibody | Cadherin 1 antibody | cadherin 1 type 1 E-cadherin antibody | Cadherin-1 antibody | Cadherin1 antibody | CAM 120/80 antibody | CD 324 antibody | CD324 antibody | CD324 antigen antibody | cdh1 antibody | CDHE antibod |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | E Cadherin |
| Immunogen | Synthetic peptide of human CDH1 (AA: DWVIPPISCPENEKC) |
| Positive Control | WB: LNCAP, A431, DU145, PC-3, MCF-7, PC-12, NIH/3T3, C6 and COS7 cell lysates. IHC: Gastric cancer tissues and Lung cancer tissues. Flow: Hela cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse monoclonal (7H12) to E Cadherin, could be used for WB, IHC and Flow. |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Storage: 4℃; -20℃ for long term storage. Avoid repeated freeze/thaw cycles. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
E Cadherin Mouse mAb (Ab100725) - Western Blot
All lanes: E Cadherin Mouse mAb (Ab100725) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 135 kDa
Observed band size: 112&124 kDa
E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human gastric tissues using E Cadherin Mouse mAb (Ab100725) with DAB staining.
E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using E Cadherin Mouse mAb (Ab100725) with DAB staining.
E Cadherin Mouse mAb (Ab100725) - Flow Cytometry
Flow cytometric analysis of HeLa cells using E Cadherin Mouse mAb (Ab100725) (green) and negative control (purple).
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| Flow Cytometry |
1/200-1/400
|
| ELISA |
1/10000
|
| IHC |
1/200-1/1000
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Jul 26, 2023 | Ab100725 | |
| Certificate of Analysis | Jul 26, 2023 | Ab100725 |
Starting at $39.90
Starting at $39.90
Starting at $109.90
Starting at $69.90