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E Cadherin Mouse mAb - Primary antibody, specific to E Cadherin, IgG1

    Application:
  • ELISA
  • Flow Cytometry
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Monkey,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 7H12
  • Conjugation: Unconjugated
In stock
Item Number
Ab100725
Grouped product items
SKU Size
Availability
Price Qty
Ab100725-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab100725-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$207.90
Ab100725-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$329.90
Ab100725-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,237.90

mAb (7H12); Mouse anti Human E Cadherin Antibody; WB, IHC, Flow, ELISA; Unconjugated

Basic Description

Product Name E Cadherin Mouse mAb - Primary antibody, specific to E Cadherin, IgG1
Synonyms Arc 1 antibody | CADH1_HUMAN antibody | Cadherin 1 antibody | cadherin 1 type 1 E-cadherin antibody | Cadherin-1 antibody | Cadherin1 antibody | CAM 120/80 antibody | CD 324 antibody | CD324 antibody | CD324 antigen antibody | cdh1 antibody | CDHE antibod
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species Mouse
Specificity E Cadherin
Immunogen Synthetic peptide of human CDH1 (AA: DWVIPPISCPENEKC)
Positive Control WB: LNCAP, A431, DU145, PC-3, MCF-7, PC-12, NIH/3T3, C6 and COS7 cell lysates. IHC: Gastric cancer tissues and Lung cancer tissues. Flow: Hela cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse monoclonal (7H12) to E Cadherin, could be used for WB, IHC and Flow.

Application
WB: 1/500-1/2000
IHC: 1/200-1/1000
Flow: 1/200-1/400
ELISA: 1/10000

Purity
Protein G purified.

Protein Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Product Properties

Isotype IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Storage: 4℃; -20℃ for long term storage. Avoid repeated freeze/thaw cycles.

Associated Targets(Human)

CDH1 Tbio Cadherin-1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

E Cadherin Mouse mAb (Ab100725) - Western Blot
All lanes: E Cadherin Mouse mAb (Ab100725) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 135 kDa
Observed band size: 112&124 kDa

E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human gastric tissues using E Cadherin Mouse mAb (Ab100725) with DAB staining.

E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using E Cadherin Mouse mAb (Ab100725) with DAB staining.

E Cadherin Mouse mAb (Ab100725) - Flow Cytometry
Flow cytometric analysis of HeLa cells using E Cadherin Mouse mAb (Ab100725) (green) and negative control (purple).

Application

Application Dilution info
WB

1/500-1/2000

Flow Cytometry

1/200-1/400

ELISA

1/10000

IHC

1/200-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0700709 Certificate of Analysis Jul 26, 2023 Ab100725
ZJ23F0700712 Certificate of Analysis Jul 26, 2023 Ab100725

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