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CD47 Mouse mAb - Primary antibody, specific to CD47, Mouse IgG1

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CD47 Mouse mAb - Primary antibody, specific to CD47, Mouse IgG1

Grade & Purity:

See COA

Application:

Flow Cytometry

Host species: Mouse
Reactive species: Human
Isotype: Mouse IgG1
Clone Number: B6H12
Conjugation: Unconjugated

In stock

Item Number

Ab094962

Grouped product items
SKU	Size	Availability	Price	Qty
Ab094962-10μg	10μg	10	$26.90
Ab094962-50μg	50μg	4	$69.90
Ab094962-100μg	100μg	2	$109.90
Ab094962-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$899.90

mAb (B6H12); Mouse anti Human CD47 Antibody; Flow; Unconjugated

View related series

Click to view target information: CD47

Accession#:Q08722 (16) CD47 (19) Flow Cytometry (1158) Gene ID:961 (16)

Basic Description

Product Name	CD47 Mouse mAb - Primary antibody, specific to CD47, Mouse IgG1
Synonyms	antigen identified by monoclonal 1D8 \| Antigenic surface determinant protein OA3 \| CD47 antigen (Rh-related antigen, integrin-associated signal transducer) \| CD47 antigen \| CD47 glycoprotein \| CD47 molecule \| CD47 \| IAP \| IAPintegrin associated protein \|
Specifications & Purity	Carrier Free, ExactAb™, Azide Free, Validated, See COA
Host species	Mouse
Specificity	CD47
Immunogen	Purified human placental RGD-binding proteins
Positive Control	Flow: Human peripheral blood lymphocytes.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human CD47 Antibody, Monoclonal (B6H12), could be used for Flow and so on. Application: Flow: 1.0 μg/100w cells Protein Function Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)

Discover Target: CD47

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Flow Cytometry: CD47 Mouse mAb (Ab094962)

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD47 with CD47 Mouse mAb (Ab094962) conjugated with biotin (right panel) at 2.0 μg/mL for 1 hour at 4°C, compared with Mouse IgG (Biotin) (Ab170221) - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD47 Mouse mAb (Ab094962)

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD47 (red) with CD47 Mouse mAb (Ab094962) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Mouse IgG (Biotin) (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD47 Mouse mAb (Ab094962)

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of MCF-7 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094962) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD47 Mouse mAb (Ab094962)

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of A549 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094962) at 5.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD47 Mouse mAb (Ab094962)

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094962) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD47 Mouse mAb (Ab094962)

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of U937 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094962) at 0.05 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	1μg/100w cells

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505965	Certificate of Analysis	Feb 25, 2025	Ab094962
ZJ24F0505966	Certificate of Analysis	Feb 25, 2025	Ab094962
ZJ24F0505964	Certificate of Analysis	May 22, 2024	Ab094962

Alternative Products

Documents & Articles

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Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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