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CD45 Rat mAb (Biotin) - Primary antibody, specific to CD45

COA COA
    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2b
  • Clone Number: I3/2.3
  • Conjugation: Biotin
In stock
Item Number
Ab185147
Grouped product items
SKU Size
Availability
 Price Qty
Ab185147-10μg
10μg
3
$19.90
Ab185147-25μg
25μg
2
$39.90
Ab185147-100μg
100μg
1
$89.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (I3/2.3); Rat anti Mouse CD45 Antibody (Biotin); Flow Cytometry

View related series
Click to view target information: PTPRC
Accession#:P06800 (28) B220 (34) CD45R (34) Flow Cytometry (1158) Gene ID:19264 (28) L-CA (34) loc (16) Ly-5 (23) Lyt-4 (16) PTPRC (24) T200 (34)

Basic Description

Product Name CD45 Rat mAb (Biotin) - Primary antibody, specific to CD45
Synonyms B220 | CD_antigen: CD45 | CD45 antigen | CD45 | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | CD45 Monoclonal Antibody (I3/2.3), Biotin | Biotin anti-
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Host species Rat
Specificity CD45
Immunogen Mouse lymphoma cell line
Positive Control Flow: BALB/C mouse splenocytes.
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Rat anti Mouse CD45 Antibody, Monoclonal (I3/2.3), could be used for Flow and so on.

Product Properties

SDS-PAGE 150 kDa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD45 Rat mAb (Biotin) (Ab185147) - Flow Cytometry
Flow cytometry analysis of CD45 expression by BALB/C mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD45 Rat mAb (Biotin) (Ab185147) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).

CD45 Rat mAb (Biotin) (Ab185147) - Flow Cytometry
Flow cytometry analysis of CD45 expression by BALB/C mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD45 Rat mAb (Biotin) (Ab185147) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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