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CD45 Rat mAb - Primary antibody, specific to CD45, Rat IgG2b

COA COA
    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2b
  • Clone Number: I3/2.3
  • Conjugation: Unconjugated
In stock
Item Number
Ab094914
Grouped product items
SKU Size
Availability
 Price Qty
Ab094914-10μg
10μg
10
$16.90
Ab094914-50μg
50μg
4
$49.90
Ab094914-100μg
100μg
2
$79.90
Ab094914-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$519.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (I3/2.3); Rat anti Mouse CD45 Antibody; Flow; Unconjugated

View related series
Click to view target information: PTPRC
Accession#:P06800 (28) CD45 (68) Flow Cytometry (1158) Gene ID:19264 (28)

Basic Description

Product Name CD45 Rat mAb - Primary antibody, specific to CD45, Rat IgG2b
Synonyms B220 | CD_antigen: CD45 | CD45 antigen | CD45 | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | PTPRC | receptor-type tyrosine-protein phosphatase C | T
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, See COA
Host species Rat
Specificity CD45
Immunogen Mouse lymphoma cell line
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, Validated
Product Description

Rat anti Mouse CD45 Antibody, Monoclonal (I3/2.3), could be used for Flow and so on.

Application

Flow: 1.0 μg/100w cells

Protein Function

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Product Properties

Isotype Rat IgG2b
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at 2-8°C
Shipped In Wet ice
Stability And Storage Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD45 Rat mAb (Ab094914) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45 with CD45 Rat mAb (Ab094914) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Isotype Control (left panel) and detected with SA-PE (rp156150).

CD45 Rat mAb (Ab094914) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45 (red) with CD45 Rat mAb (Ab094914) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

1μg/100w cells

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0404998 Certificate of Analysis Apr 29, 2024 Ab094914
ZJ24F0404997 Certificate of Analysis Apr 29, 2024 Ab094914
ZJ24F0404996 Certificate of Analysis Apr 29, 2024 Ab094914

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