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CD45 Mouse mAb - Primary antibody, specific to CD45, Mouse IgG1

COA COA
    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: 2D1
  • Conjugation: Unconjugated
In stock
Item Number
Ab094898
Grouped product items
SKU Size
Availability
 Price Qty
Ab094898-10μg
10μg
10
$26.90
Ab094898-50μg
50μg
4
$69.90
Ab094898-100μg
100μg
2
$99.90
Ab094898-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$899.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (2D1); Mouse anti Human CD45 Antibody; Flow; Unconjugated

View related series
Click to view target information: PTPRC
Accession#:P08575 (56) CD45 (68) Flow Cytometry (1158) Gene ID:5788 (52)

Basic Description

Product Name CD45 Mouse mAb - Primary antibody, specific to CD45, Mouse IgG1
Synonyms B220 | CD_antigen: CD45 | CD45 antigen | CD45 | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | PTPRC | receptor-type tyrosine-protein phosphatase C | T
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, See COA
Host species Mouse
Specificity CD45
Immunogen Human PBMC
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human CD45 Antibody, Monoclonal (2D1), could be used for Flow and so on.

Application

Flow: 1.0 μg/100w cells

Protein Function

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at 2-8°C
Shipped In Wet ice
Stability And Storage Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD45 Mouse mAb (Ab094898) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094898) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD45 Mouse mAb (Ab094898) - Flow Cytometry
Flow Cytometry analysis of THP-1 cells labelling CD45 (red) with CD45 Mouse mAb (Ab094898) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD45 Mouse mAb (Ab094898) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 with CD45 Mouse mAb (Ab094898) conjugated with biotin (right panel) at 2 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

CD45 Mouse mAb (Ab094898) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 (red) with CD45 Mouse mAb (Ab094898) conjugated with biotin at 2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

1μg/100w cells

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0404666 Certificate of Analysis Apr 19, 2024 Ab094898
ZJ24F0404665 Certificate of Analysis Apr 19, 2024 Ab094898
ZJ24F0404664 Certificate of Analysis Apr 19, 2024 Ab094898

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