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CD25 Rat mAb (PE-Cy5) - Primary antibody, specific to CD25

    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG1
  • Clone Number: PC-61.5.3
  • Conjugation: PE-Cy5
In stock
Item Number
Ab211662
Grouped product items
SKU Size
Availability
Price Qty
Ab211662-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab211662-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab211662-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$239.90

mAb (PC-61.5.3); Rat anti Mouse CD25 Antibody (PE-Cy5); Flow Cytometry

Basic Description

Product Name CD25 Rat mAb (PE-Cy5) - Primary antibody, specific to CD25
Synonyms Anti-mouse CD25 (IL-2Rα)-InVivo | In Vivo MAb anti-mouse CD25 (IL-2Rα) | CD25 Rat mAb | CD25 | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2 receptor sub
Specifications & Purity ExactAb™, Validated, Ex:565nm, Em:670nm, 0.5 mg/mL
Host species Rat
Specificity CD25
Immunogen IL-2-dependent cytolytic mouse T cell clone B6.1
Positive Control Flow: C57BL/6 mouse splenocytes
Conjugation PE-Cy5
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 670nm
Ex/Em(nm) Ex:565nm;Em:670nm
Grade Em:670nm, Ex:565nm, ExactAb™, Validated
Product Description

Rat anti Mouse CD25 Antibody, Monoclonal (PC-61.5.3), could be used for Flow Cytometry

Product Properties

Light Chain Type lambda
Purification Method Protein A purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD25 Rat mAb (PE-Cy5) (Ab211662) - Flow Cytometry
Con A-stimulated (2 days) C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells (right panel) were then stained with CD25 Rat mAb (PE-Cy5) (Ab211662) (red) compared to an isotype control (blue). Blue - Rat IgG (PE-Cy5) (Ab190072). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

Application

Application Dilution info
Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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