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CD18 Rat mAb (Biotin) - Primary antibody, specific to CD18

COA COA
    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: M18/2
  • Conjugation: Biotin
In stock
Item Number
Ab189639
Grouped product items
SKU Size
Availability
 Price Qty
Ab189639-10μg
10μg
3
$19.90
Ab189639-25μg
25μg
2
$36.90
Ab189639-100μg
100μg
1
$79.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (M18/2); Rat anti Mouse CD18 Antibody (Biotin); Flow Cytometry

View related series
Click to view target information: ITGB2
2E6 (2) Accession#: P11835 (3) CD18 (6) Flow Cytometry (1158) Gene ID: 16414 (3) LAD (2) LCAMB (2) Lfa1 (2) MF17 (2)

Basic Description

Product Name CD18 Rat mAb (Biotin) - Primary antibody, specific to CD18
Synonyms Cd18 | Lfa1 | 959 beta subunit precursor)10 | CD18 antigen | CD18beta 2 | cell surface adhesion glycoprotein (LFA-1 | CR3 | Integrin beta 2 | integrin beta-2 | integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1 | macrophage ant
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence
Host species Rat
Specificity CD18
Immunogen C57BL/10 splenocytes
Positive Control Flow: BALB/C mouse splenocytes.
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Rat anti Mouse CD18 Antibody, Monoclonal (M18/2), could be used for in vivo LFA-1 neutralization, Flow and so on.

Product Properties

Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

ITGB2 Tclin Integrin beta-2 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
ITGB2 Tclin Integrin beta-2 (0 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
ITGB2 Tclin Integrin alpha-M/beta-2 (32 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD18 Rat mAb (Biotin) (Ab189639) - Flow Cytometry
Flow cytometry analysis of CD18 expression by BALB/C mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD18 Rat mAb (Biotin) (Ab189639) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310). 

CD18 Rat mAb (Biotin) (Ab189639) - Flow Cytometry
Flow cytometry analysis of CD18 expression by BALB/C mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD18 Rat mAb (Biotin) (Ab189639) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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