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Anti-Kindlin 2 antibody - Primary antibody, specific to FERMT2

    Application:
  • IF/ICC
  • IHC
  • IP
  • WB
  • Host species: Mouse
  • Reactive species: Dog,Human,Monkey,Mouse,Rat
  • Isotype: Mouse IgG2a
  • Clone Number: OTI14A11
  • Conjugation: Unconjugated
In stock
Item Number
Ab112146
Grouped product items
SKU Size
Availability
Price Qty
Ab112146-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab112146-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab112146-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab112146-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

mAb (OTI14A11); Mouse anti Human Kindlin 2 Antibody; WB,IHC, IF/ICC, IP; Unconjugated

Basic Description

Product Name Anti-Kindlin 2 antibody - Primary antibody, specific to FERMT2
Synonyms FLJ34213 | FLJ44462 | UNC112 | FERM2_HUMAN | Fermitin family homolog 2 | Fermt2 | KIND2 | Kindlin-2 | MIG-2 | MIG2 | Mitogen-inducible gene 2 protein | PH domain-containing family C member 1 | Pleckstrin homology domain containing family C (with FERM doma
Specifications & Purity KO Validation, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
Host species Mouse
Specificity FERMT2
Immunogen Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector
Positive Control WB: Hep G2, HeLa, A549, NIH/3T3 and C6 cell lysates. IHC: Human pancreas tissues. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY FERMT2, Hep G2 cells. IP: HEK293T lysates.
Conjugation Unconjugated
Grade ExactAb™, KO Validation, Validated
Product Description

Mouse anti Human Kindlin 2 Antibody, Monoclonal (OTI14A11), could be used for WB, IHC, IF/ICC, IP and so on.

Product Properties

SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

FERMT2 Tbio Fermitin family homolog 2 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Kindlin 2 Mouse mAb (Ab112146) - Western Blot
All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/30000 dilution

Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 100.0 s

Kindlin 2 Mouse mAb (Ab112146) - Western Blot
All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 30.0 s

Kindlin 2 Mouse mAb (Ab112146) - KO
Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT) and FERMT2-Knockout HeLa cells (KO) were separated by SDSPAGE and immunoblotted with Kindlin 2 Mouse mAb (Ab112146) (1/500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

Kindlin 2 Mouse mAb (Ab112146) - IF/ICC
Kindlin 2 Mouse mAb (Ab112146) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2.


Kindlin 2 Mouse mAb (Ab112146) - IF/ICC
Immunofluorescent staining of Hep G2 cells using Kindlin 2 Mouse mAb (Ab112146).

Kindlin 2 Mouse mAb (Ab112146) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using Kindlin 2 Mouse mAb (Ab112146). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min).

Application

Application Dilution info
WB

1/1000-1/2000

IF/ICC

1/50-1/100

IHC

1/50

IP

2ug/500ul

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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