Skip to the beginning of the images gallery

5-Carboxyfluorescein - 95%, high purity , CAS No.76823-03-5

14 Citations

COA

Grade & Purity:

≥95%

Cas Number: 76823-03-5
Molecular Weight: 376.32
Beilstein Registry Number: 57037
MDL number: MFCD00036874
PubChem CID: 123755
PubChem SID: 488187777

In stock

Item Number

C105530

Grouped product items
SKU	Size	Availability	Price
C105530-25mg	25mg	5	$17.90
C105530-100mg	100mg	5	$58.90
C105530-250mg	250mg	5	$88.90
C105530-500mg	500mg	5	$117.90
C105530-1g	1g	5	$212.90
C105530-5g	5g	1	$955.90
C105530-25g	25g	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$4,301.90

Amine-reactive fluorescent label

View related series

Fluorescent Dye (372) Six-Membered Heterocyclic Compounds (1696) Spectroscopic reagent (233)

Basic Description

Synonyms	Q27122687 \| 3',6'-Dihydroxy-3-oxo-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid \| BCP22613 \| DTXSID30227642 \| 5-FAM \| 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid \| AS-32304 \| PD012213 \| Spiro(isobenzofuran-1(3H),9'-(9H)
Specifications & Purity	≥95%
Biochemical and Physiological Mechanisms	Amine-reactive fluorescent label. 6-FAM isomer. Polar tracer. Covalently labels peptides and proteins. 5-CFDA N-succinimidyl ester and 5-CFDA hydrolytic product.
Storage Temp	Store at 2-8°C
Shipped In	Wet ice This product requires cold chain shipping. Ground and other economy services are not available.
Note	Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description	5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Organoheterocyclic compounds
  - Class Benzopyrans
    - Subclass 1-benzopyrans
      - Level5 Dibenzopyrans
        Level6 Xanthenes

Kingdom	Organic compounds
Superclass	Organoheterocyclic compounds
Class	Benzopyrans
Subclass	1-benzopyrans
Intermediate Tree Nodes	Dibenzopyrans
Direct Parent	Xanthenes
Alternative Parents	M-phthalic acid and derivatives Diarylethers Phthalides Benzofuranones Isobenzofurans 1-hydroxy-2-unsubstituted benzenoids Dicarboxylic acids and derivatives Lactones Carboxylic acid esters Oxacyclic compounds Carboxylic acids Organic oxides Hydrocarbon derivatives
Molecular Framework	Aromatic heteropolycyclic compounds
Substituents	Xanthene - Meta_phthalic_acid - Diaryl ether - Benzofuranone - Phthalide - Isobenzofuranone - Isobenzofuran - Isocoumaran - 1-hydroxy-2-unsubstituted benzenoid - Benzenoid - Dicarboxylic acid or derivatives - Lactone - Carboxylic acid ester - Oxacycle - Ether - Carboxylic acid - Carboxylic acid derivative - Hydrocarbon derivative - Organic oxide - Organooxygen compound - Organic oxygen compound - Aromatic heteropolycyclic compound
Description	This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
External Descriptors	monocarboxylic acid
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Names and Identifiers

Pubchem Sid	488187777
Pubchem Sid Url	https://pubchem.ncbi.nlm.nih.gov/substance/488187777
IUPAC Name	3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
INCHI	InChI=1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
InChIKey	NJYVEMPWNAYQQN-UHFFFAOYSA-N
Smiles	C1=CC2=C(C=C1C(=O)O)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
Isomeric SMILES	C1=CC2=C(C=C1C(=O)O)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
WGK Germany	3
Molecular Weight	376.32
Beilstein	57037
Reaxy-Rn	57037
Reaxys-RN_link_address	https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=57037&ln=

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

25 results found

Lot Number	Certificate Type	Date	Item
K2011125	Certificate of Analysis	Aug 16, 2024	C105530
D2403383	Certificate of Analysis	Mar 08, 2024	C105530
F1928038	Certificate of Analysis	Apr 13, 2023	C105530
C2329688	Certificate of Analysis	Feb 21, 2023	C105530
C2329692	Certificate of Analysis	Feb 21, 2023	C105530
C2329674	Certificate of Analysis	Feb 21, 2023	C105530
C2329676	Certificate of Analysis	Feb 21, 2023	C105530
C2329677	Certificate of Analysis	Feb 21, 2023	C105530
C2329691	Certificate of Analysis	Feb 21, 2023	C105530
C2329678	Certificate of Analysis	Feb 21, 2023	C105530
C2329689	Certificate of Analysis	Feb 21, 2023	C105530
C2329679	Certificate of Analysis	Feb 21, 2023	C105530
C2329680	Certificate of Analysis	Feb 21, 2023	C105530
C2329690	Certificate of Analysis	Feb 21, 2023	C105530
K1424008	Certificate of Analysis	Aug 09, 2022	C105530
I1814032	Certificate of Analysis	Jul 18, 2022	C105530
B2211302	Certificate of Analysis	Dec 20, 2021	C105530
B2211376	Certificate of Analysis	Dec 20, 2021	C105530
B2211219	Certificate of Analysis	Dec 20, 2021	C105530
B2211301	Certificate of Analysis	Dec 20, 2021	C105530
B2211299	Certificate of Analysis	Dec 20, 2021	C105530
B2211297	Certificate of Analysis	Dec 20, 2021	C105530
J2307063	Certificate of Analysis	Dec 20, 2021	C105530
J2424309	Certificate of Analysis	Dec 20, 2021	C105530
H2419103	Certificate of Analysis	Dec 20, 2021	C105530

Show more⌵

Chemical and Physical Properties

Solubility	soluble in DMF and H2O and DMSO
Sensitivity	light & Moisture sensitive
Molecular Weight	376.300 g/mol
XLogP3	2.900
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	1
Exact Mass	376.058 Da
Monoisotopic Mass	376.058 Da
Topological Polar Surface Area	113.000 Å²
Heavy Atom Count	28
Formal Charge	0
Complexity	637.000
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
The total count of all stereochemical bonds	0
Covalently-Bonded Unit Count	1

Documents & Articles

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration