Thiazolyl blue | 298-93-1 | Thiazolyl blue tetrazolium bromide | MTT | MMT Tetrazolium | Methylthiazoletetrazolium | 2348-71-2 | Thiazolyl Blue Monotetrazolium | 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide | 3-(4,5-Dimethylthiazolyl)-2,5-diphenyltetrazoliu
Specifications & Purity
10mM in DMSO
Biochemical and Physiological Mechanisms
Membrane-permeable dye used in measurement of cell proliferation. It produces a yellowish tetrazole solution that is converted to dark blue, water-insoluble MTT formazan by intracellular NAD(P)H-oxidoreductases of living cells.
Storage Temp
Protected from light,Store at -80°C
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Product Description
Thiazolyl Blue is a membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT Formazan). Spectrophotometric measurement of MTT Formazan at 540 or 570 nm allows quantitation of cell viability. Thiazolyl Blue is used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. Thiazolyl Blue is also an electron acceptor used for studying NADP-dependent dehydrogenases. A membrane-permeable dye reduced by mitochondrial enzymes
This compound belongs to the class of organic compounds known as phenyltetrazoles and derivatives. These are compounds containing a phenyltetrazole skeleton, which consists of a tetrazole bound to a phenyl group.
External Descriptors
organic bromide salt
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
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