This is a demo store. No orders will be fulfilled.

Z-DEVD-AMC

  • 分子式: C36H41N5O14
  • 分子量: 767.74
有货

库存信息

关闭

库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
Z658857-5mg
5mg 现货 Stock Image
Z658857-25mg
25mg 现货 Stock Image

基本描述

别名 剛果紫外光學
规格或纯度 BioReagent, ≥98%
稳定性与储存 长期储存-20℃(24个月); 复溶后建议分装,避免反复冻融;避光;密封。
英文名称 Z-DEVD-AMC
储存温度 避光,-20°C储存,干燥,避免反复冻融
运输条件 超低温冰袋运输
产品介绍

Z-DEVD-AMC 是一种经典的Caspase-3/7底物,用于在体外或细胞裂解体系中检测其酶活性。该分子由四肽序列DEVD(Caspase-3/7的特异识别序列)和荧光团AMC(7-氨基-4-甲基香豆素)组成。正常情况下,AMC被DEVD序列封闭,不发光;当Caspase-3或-7被激活后,会识别并切割DEVD与AMC之间的键,释放出自由的AMC分子,从而发出可被检测的蓝色荧光(Ex/Em ≈ 350/450 nm)。

Z-DEVD-AMC常用于比色板荧光酶活检测、药物诱导凋亡的研究、高通量药筛系统中等,尤其适合进行Caspase活性曲线分析和时间依赖性实验。其优点包括反应速度快、信号强、背景低,但由于激发波长较短(UV区),推荐使用具备紫外激发通道的荧光酶标仪或荧光分光光度计。此外,也可用于裂解细胞后进行荧光定量检测,不适用于活细胞直接成像。操作时注意避免强光照射,以减少AMC的自发光衰减。

Z-DEVD-AMC is a classic Caspase-3/7 substrate, used to detect the enzymatic activity of Caspase-3/7 in vitro or in cell lysis systems. This molecule consists of a tetrapeptide sequence DEVD (a specific recognition sequence for Caspase-3/7) and a fluorophore AMC (7-amino-4-methylcoumarin). Under normal circumstances, AMC is blocked by the DEVD sequence and does not emit light; when Caspase-3 or -7 is activated, it will recognize and cleave the bond between DEVD and AMC, releasing free AMC molecules, which then emit detectable blue fluorescence (Ex/Em ≈ 350/450 nm).
Z-DEVD-AMC is often used in microplate fluorescent enzyme activity assays, studies on drug-induced apoptosis, high-throughput drug screening systems, etc., and is particularly suitable for Caspase activity curve analysis and time-dependent experiments. Its advantages include fast reaction speed, strong signal, and low background. However, due to its relatively short excitation wavelength (in the UV region), it is recommended to use a fluorescence microplate reader or fluorescence spectrophotometer equipped with an ultraviolet excitation channel. In addition, it can also be used for fluorescent quantitative detection after cell lysis, but is not suitable for direct imaging of living cells. During operation, attention should be paid to avoiding strong light exposure to reduce the self-luminescence attenuation of AMC.

纯度 ≥98%

名称和识别符

分子量 767.74

化学和物理性质

溶解性 DMSO : 33.33 mg/mL (43.41 mM; Need ultrasonic)

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到2个结果

批号(Lot Number) 证书类型 货号
ZJ25F1028578 分析证书 Z658857
ZJ25F1028577 分析证书 Z658857

可替换产品

溶液计算器