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| 产品名称 | 链霉亲和素 |
|---|---|
| 别名 | 抗生物素蛋白 | 抗生物素蛋白链霉菌 |
| 英文别名 | Streptavidin |
| 规格或纯度 | BioReagent, 含保护剂 |
| 生化机理 | 链霉亲和素是一种生物素结合蛋白，能结合四种生物素分子。链霉亲和素的生物素结合口袋有助于与生物素相互作用。它可以用来增强蛋白质结合和多聚化。 |
| 产品介绍 |
链霉抗生物素蛋白是一个非糖基化的蛋白质。 它对生物素具有优异的高亲和力，并且在大幅度的pH和温度范围内稳定。 由于其对生物素的高亲和力，它被用来桥接生物素化的分子。 每个链霉亲和素分子可以结合4个生物素分子。链霉亲和素是一种来源于阿维丁链霉菌（streptomyces avidinii）的生物素结合蛋白质，其非特异性结合远比亲和素低，链霉亲和素蛋白具有在中性pH值几乎没有净电荷，不含有碳水化合物等更有利的化学性质，所以它被广泛用来作为抗生物素蛋白的替代品。 在结构上，链霉亲和素含SA 的127AA 最佳核心结构，以同源四聚体的形式存在，每摩尔的四聚体分子可结合四摩尔的生物素分子，国际标准比活性12-15 IU/mg。该特性联合生物素偶联抗原/抗体检测技术，使其广泛应用于免疫学中ELISA检测信号的放大。此外，本品主要可用于免疫微孔板、免疫层析硝酸纤维素膜，基因芯片、蛋白芯片的表面包被，快速固定生物素化的抗原、抗体、核酸等物质。也可用于偶联NHS 磁性磁珠、树脂微球，作为发光诊断、生物素化物质分离、磁性试纸条免疫层析等方面的应用。高亲和力生物素结合蛋白（Kd 约为10-15），其在宽的pH和温度范围内可保持稳定。每个链霉亲和素分子可结合4个生物素分子。链霉亲和素缺乏存在于亲和素上的碳水化合物侧链，并具有接近中性的等电点（pI为5.5-6.5，亲和素为10）。因此，链霉亲和素通常显示出比亲和素更低的非特异性结合水平。1％溶液在280 nm处的消光系数为32。高亲和力的生物素结合蛋白。 产品组成： 冷冻干粉(5mM磷酸钾盐缓冲液、海藻糖，pH8.0)，添加保护剂改进了产品溶解性问题和沉淀问题
Streptavidin from Streptomyces avidinii has been used: in the pre-functionalization of self-propelled catalytic micromotors in the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substrates as an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC) Streptavidin is used as a tetrameric protein used to visualize biotin conjugated molecules in ELISA. Due to the strong affinity of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin is widely used in molecular biology through its unique high affinity for the biotin. |
| 偶联 | Unconjugated |
储存与运输
| 浓度 | 含保护剂 |
|---|---|
| 储存温度 | -20°C储存,充氩 |
| 运输条件 | 超低温冰袋运输 |
| CAS编号和信息 | 9013-20-1 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 G2511763 |
分析证书 | S759021 |
技术文档和文章
生物素-亲和素系统：蛋白研究的黄金工具
Categories: 技术文章
此产品的引用文献
引用文献
| 1. Yuping Liu, Beibei Zhang, Xueyuan Wu, Fan Wang, Zhiyi Yang, Mengyi Li, Kaixuan Sheng, Yue Yan, Liang Zhu, Hui Jing, Yanmin Wu, Lili Hu, Yanyan Yu, Chenglin Li. (2025) A facile liquid biopsy assay for highly efficient CTCs capture and reagent-less monitoring of immune checkpoint PD-L1 expression on CTCs with non-small cell lung cancer patients. BIOSENSORS & BIOELECTRONICS, 275 (117236). [PMID:39929085] [10.1016/j.bios.2025.117236] |
| 2. Xiaohui Yang, Yixian Li, Josh Zixi Lee, Yuanmin Sun, Xin Tan, Yijie Liu, Yang Yu, Huiqiang Li, Xue Li. (2024) A Highly Sensitive Dual-Drive Microfluidic Device for Multiplexed Detection of Respiratory Virus Antigens. Micromachines, 15 (6): (685). [PMID:38930655] [10.3390/mi15060685] |
| 3. Ziyang He, Yonghong Meng, Mei Liu. (2024) A multicolor aptasensor based on target-induced anti-etching gold nanorod for visible detection of aflatoxin B1. MICROCHEMICAL JOURNAL, 201 (110659). [10.1016/j.microc.2024.110659] |
| 4. Yunzhi Zhao, Ying Hao, Min Cui, Na Li, Bao Sun, Yu Wang, Haiyan Zhao, Cong Zhang. (2024) An electrochemical biosensor based on DNA tetrahedron nanoprobe for sensitive and selective detection of doxorubicin. BIOELECTROCHEMISTRY, 157 (108652). [PMID:38271768] [10.1016/j.bioelechem.2024.108652] |
| 5. Jiawei Chen, Gan Zhang, Xiaoyue Xiao, Daofeng Liu, Juan Peng, Yonghua Xiong, Weihua Lai. (2024) Bifunctional bovine serum albumin modification driven sensitivity-enhanced lateral flow immunoassay for small molecule hazards monitoring in food. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, (136915). [PMID:39476895] [10.1016/j.ijbiomac.2024.136915] |
| 6. Yangyang Li, Riyue Shu, Cheng Zeng, Jing Wang, Lin Zhang, Zhe Tang. (2025) Bio-inspired AS1411-Functionalized membrane for specific and non-destructive capture of tumor cells. JOURNAL OF MEMBRANE SCIENCE, 721 (123833). [10.1016/j.memsci.2025.123833] |
| 7. Danyu Wang, Xin Zhou, Mengyu Huang, Jie Duan, Yue Qiu, Hua Yi, Yang Wang, Huimin Xue, Jiali Zhang, Qiuxia Yang, Hua Gao, Zhenzhen Guo, Kaixiang Zhang. (2024) Cascade Enzymes Confined in DNA Nanoanchors for Antitumor Therapy. ACS Applied Materials & Interfaces, 16 (38): (50295-50304). [PMID:39265065] [10.1021/acsami.4c09835] |
| 8. Huinan Chen, Jingyao Song, Yuanyuan Li, Dongmei Deng, Yuchen Song, Xiaoli Zhu, Liqiang Luo. (2024) Cascade signal amplifying strategy for ultrasensitive detection of tumor biomarker by DNAzyme cleaving mediated HCR. SENSORS AND ACTUATORS B-CHEMICAL, 420 (136466). [10.1016/j.snb.2024.136466] |
| 9. Junqiu Liu, Zhaidong Liu, Chunqin Zhao, Yuting Jiao, Baohong Li, Jiaju Shi, Zichao Chen, Zhen Zhang. (2024) Coaxial dual-path electrochemical biosensing and logic strategy-based detection of lung cancer-derived exosomal PD-L1. Nanoscale, [PMID:38630023] [10.1039/D4NR00412D] |
| 10. Boyu Yang, Chao Li, Yongshuo Ren, Weichen Wang, Xiangxiang Zhang, Xiaojun Han. (2024) Construction of the Glycolysis Metabolic Pathway Inside an Artificial Cell for the Synthesis of Amino Acid and Its Reversible Deformation. Journal of the American Chemical Society, 146 (31): (21847-21858). [PMID:39042264] [10.1021/jacs.4c06227] |
| 11. Yan Liu, Yujie Li, Chen Shao, Ping Wang, Xiaoxuan Wang, Runhe Li. (2024) Curcumin-based residue-free and reusable photodynamic inactivation system for liquid foods and its application in freshly squeezed orange juice. FOOD CHEMISTRY, 458 (140316). [PMID:38968711] [10.1016/j.foodchem.2024.140316] |
| 12. Qiudi Xu, Ke Liu, Yu He, Lan Wang, Zefan Lu, Zhongxuan Liu, Tao Zhang. (2025) Digital microfluidic chip with photopatterned reactive sites for direct biomolecules immobilization and magnetic beads-free immunoassay. SENSORS AND ACTUATORS B-CHEMICAL, 424 (136893). [10.1016/j.snb.2024.136893] |
| 13. Hang Ao, Wencheng Xiao, Wenrui Hu, Jie Wu, Huangxian Ju. (2024) DNA Conformation-Regulated Hemin Switch for Lab-on-Chip Chemiluminescent Detection of an Antibody Secreted from Hybridoma Cells. ANALYTICAL CHEMISTRY, 96 (46): (18502-18509). [PMID:39503400] [10.1021/acs.analchem.4c04122] |
| 14. Mingxia Lin, Huiyi Yang, Qinglan Li, Huanxin Xiao, Shilin Jiang, Jinhui Liang, Xiping Cui, Suqing Zhao. (2024) Dual lateral flow assay based on PdRu nanocages for human Papillomavirus detection. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 673 (893). [PMID:38908288] [10.1016/j.jcis.2024.06.002] |
| 15. Rongsheng Hu, Shaowei Lin, Fajun Li, Jiaqing Shen, Yinong Xie, Baichang Deng, Youyu Zhang, Zhaogang Dong, Jinfeng Zhu. (2024) Exploring Aptamer-Based Metasurfaces for Label-Free Plasmonic Biosensing of Breast Tumor-Derived Exosomes. Advanced Optical Materials, 12 (28): (2401180). [10.1002/adom.202401180] |
| 16. Shihong Zhu, Daohang Du, Zhimin Zhao, Xianfeng Chu, Daoxiang Su, Shuli Yu, Ting Tao, Yong Jiang, Zhifei Wang. (2025) Fabrication of functional interface on magnetic beads via various amino acids and their application in chemiluminescent immunoassay as carrier. COLLOIDS AND SURFACES B-BIOINTERFACES, 245 (114364). [PMID:39527882] [10.1016/j.colsurfb.2024.114364] |
| 17. Danyi Zhao, Mai Hu, Chenghao Hu, Di Wang, Hailun Chen, Yangwei Ou, Rongli Liu, Xiaoyang Li, Long Wu, Peng Liu, Zhiwei Shen, Qi Chen. (2025) Multivalent bifunctional nanobody to enhance the sensitivity of direct competitive chemiluminescence immunoassay for the detection of microcystin LR in lake water. TALANTA, 283 (127080). [PMID:39467444] [10.1016/j.talanta.2024.127080] |
| 18. Yu-E Gao, Qing Huang, Yue Huang, Li-Xia Yan, Wen-Long Wang, WenFeng Zhao, Yong-Wei Feng, Xiao-Dong Huang, Yi Zhang. (2024) Nanoparticle-mediated light-driven LAMP combined with test strips for sensitive and rapid visual detection of antibiotic resistance genes. JOURNAL OF HAZARDOUS MATERIALS, (136981). [PMID:39752829] [10.1016/j.jhazmat.2024.136981] |
| 19. Wenli Zhu, Miao Hu, Guanghua Su, Weifang Jiang, Hancong Du, Kaxi Chen, Jinfang Nie, Lang Zhang, Xuehui Tang, Yun Zhang. (2025) Nanoprobe-free nanosensor with Tyndall-effect readout for colorimetric detection of alkaline phosphatase. MICROCHEMICAL JOURNAL, 212 (113295). [10.1016/j.microc.2025.113295] |
| 20. Liping Wu, Min Qing, Zhaode Mu, Jing Zhou, Jianli Zuo, Lijuan Bai. (2024) New electrocatalyst of Ni6MnO8@AB/PtNPs with efficient synergistic enhancement in electrochemical aptasensor for sensitive detection of neuron specific enolase. MICROCHEMICAL JOURNAL, 201 (110639). [10.1016/j.microc.2024.110639] |
| 21. Huanxin Xiao, Weiguang Chen, Mingxia Lin, Shilin Jiang, Xiping Cui, Suqing Zhao. (2024) Rapid immunoassay for dual-mode detection of HPV16 and HPV18 DNA based on Au@PdPt nanoparticles. Analytical Methods, [PMID:38463013] [10.1039/D3AY02307A] |
| 22. Jianjian Ji, Yingcai Xiong, Keyu Tao, Tao Li, Weiying Ou, Yinghui Zhou, Wenyang Zhang, Ruogu Qi, Shouchuan Wang. (2024) Resveratrol inhibits respiratory syncytial virus replication by targeting heparan sulfate proteoglycan. Food & Function, [PMID:38270052] [10.1039/D3FO05131E] |
| 23. Ziru Feng, Mengmeng Yan, Tengfei Li, Wenjun Zhang, Chao Zhu. (2025) Screening specific aptamer pairs towards bovine lactoferrin for its sandwich application. Food Bioscience, 64 (105911). [10.1016/j.fbio.2025.105911] |
| 24. Jialuo Ai, Yixuan Huang, Zhaoyi Yin, Yingshan Deng, Ling Yan, Jingwen Liao, Guoyan Liang, Chong Chen, Yunbing Chang, Cairong Xiao, Jiale Zhou, Zurong Zhu, Chengli Liu, Zhuo Jiang, Chengyun Ning, Zhengao Wang. (2024) SEA Anemone-inspired Conducting Polymer Sensing Platform for Integrated Detection of Tumor Protein Marker and Circulating Tumor Cell. Advanced Healthcare Materials, (2401305). [PMID:38767216] [10.1002/adhm.202401305] |
| 25. Yi Zhang, Jing Cao, Jie Zhou, Wen-Long Wang, Zhenghua Xu, Yong-Wei Feng, Xiao-Dong Huang, Feng-De Yu, Xin Yang. (2024) Strand displacement amplification combining photothermal-colorimetric dual mode test strip for ultra-sensitive detection of patulin. MICROCHEMICAL JOURNAL, 207 (112109). [10.1016/j.microc.2024.112109] |
| 26. Qiang Zhang, Junlei Zhao, Anqi Han, Xiaonan Zhang, Mingya Yang, Hui Li, Benli Yu, Guosheng Zhang, Sheng Zhou. (2024) Ultrasensitive cancer cell sensing based on tapered optical fiber operating near the dispersion turning point. SENSORS AND ACTUATORS B-CHEMICAL, 407 (135473). [10.1016/j.snb.2024.135473] |