Recombinant Human IL-18 Protein

COA

级别和纯度:

无载体

生物活性

ActiBioPure™

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: Q14116
蛋白标签: No tag
生物活性: Immobilized Recombinant Human IL-18 Bpa Protein (rp169678) at 2.0 μg/mL can bind Recombinant Human IL-18 Protein (rp186564) with the EC50 of 30.0 ng/mL. Immobilized Recombinant IL-18 protein (rp186564) at 1.0 μg/mL can bind Camoteskimab (anti-IL-18) (Ab182692) with the EC50 of 400.2 ng/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp186564-10μg	10μg	现货
rp186564-50μg	50μg	期货
rp186564-100μg	100μg	期货
rp186564-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IL18

Accession#:Q14116 (9) Gene ID:3606 (9) IL-18 (4) IL18 (10)

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基本描述

产品名称	Recombinant Human IL-18 Protein
别名	重组人IL-18蛋白 \| 重组人白介素-18蛋白
英文别名	Iboctadekin \| IFN-gamma-inducing factor \| IGIF \| IGIFIL-1 gamma \| IL18 \| IL-18 \| IL-18MGC12320 \| IL-1F4 \| IL1F4iboctadekin \| IL-1g \| Interferon gamma-inducing factor \| interleukin 18 (interferon-gamma-inducing factor) \| Interleukin-1 gamma \| interleukin-1
规格或纯度	ActiBioPure™, 无载体, 生物活性, ≥95%(SDS-PAGE)
生化机理	Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA. Post-translational: The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form. The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor. Processing by CASP1, CASP4 or CASP5 induces conformational changes to generate critical receptor-binding sites. The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, cleavage by CASP3 inactivates IL18.
生物活性	Immobilized Recombinant Human IL-18 Bpa Protein (rp169678) at 2.0 μg/mL can bind Recombinant Human IL-18 Protein (rp186564) with the EC50 of 30.0 ng/mL. Immobilized Recombinant IL-18 protein (rp186564) at 1.0 μg/mL can bind Camoteskimab (anti-IL-18) (Ab182692) with the EC50 of 400.2 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	37-193 aa
序列	YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED
蛋白标签	No tag
无载体	Yes
无动物源	No
Accession #	Q14116
来源	重组表达
预测分子量	18.2 kDa
SDS-PAGE	15.7 kDa, under reducing conditions; 15.7 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, 2mM DTT, 8% Trehalose, 5% Mannitol, pH 7.4.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C for 1 year. Avoid freeze/thaw cycle.
分子类型	蛋白质

图片

Binding Activity: Recombinant Human IL-18 Protein (rp186564)

Recombinant Human IL-18 Protein (rp186564) - Binding Activity
Immobilized Recombinant Human IL-18 Bpa Protein (rp169678) at 2.0 μg/mL can bind Recombinant Human IL-18 Protein (rp186564) with the EC ₅₀ of 30.0 ng/mL.

Binding Activity: Recombinant Human IL-18 Protein (rp186564)

Recombinant Human IL-18 Protein (rp186564) - Binding Activity
Immobilized Recombinant IL-18 protein (rp186564) at 1.0 μg/mL can bind Camoteskimab (anti-IL-18) (Ab182692) with the EC ₅₀ of 400.2 ng/mL.

SDS-PAGE: Recombinant Human IL-18 Protein (rp186564)

Recombinant Human IL-18 Protein (rp186564) - SDS-PAGE
3 μg/lane of Recombinant Human IL-18 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 15.7 kDa.

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找到6个结果

批号(Lot Number)	证书类型	货号
ZJ25F1028480	分析证书	rp186564
ZJ25F1028479	分析证书	rp186564
ZJ25F1028478	分析证书	rp186564
ZJ24F1114408	分析证书	rp186564
ZJ24F1114407	分析证书	rp186564
ZJ24F1114406	分析证书	rp186564

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Recombinant Human IL-18 Protein

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基本描述

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