Recombinant Human IL-18 Protein

COA

级别和纯度:

≥95%(SDS-PAGE&HPLC)

表达系统: E. coli
Accession #: Q14116
蛋白标签: No tag
生物活性: 1、Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 5 - 30 ng/mL. 2、Recombinant Human IL-18 Protein (rp147444) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED50 for this effect is typically 0.27 ng/mL.
内毒素水平: <10 EU/mg

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货号 (SKU)	包装规格	是否现货
rp147444-10μg	10μg	现货
rp147444-50μg	50μg	现货
rp147444-100μg	100μg	现货
rp147444-1mg	1mg	期货

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点击查看蛋白信息: IL18

Accession#:Q14116 (9) Gene ID:3606 (9) IL18 (10)

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基本描述

产品名称	Recombinant Human IL-18 Protein
别名	重组人白介素18(IL18)蛋白 \| 重组人IL-18蛋白 \| 重组人 IL-18 蛋白
英文别名	Iboctadekin \| IFN-gamma-inducing factor \| IGIF \| IGIFIL-1 gamma \| IL18 \| IL-18 \| IL-18MGC12320 \| IL-1F4 \| IL1F4iboctadekin \| IL-1g \| Interferon gamma-inducing factor \| interleukin 18 (interferon-gamma-inducing factor) \| Interleukin-1 gamma \| interleukin-1
规格或纯度	无动物源, 无载体, 生物活性, ActiBioPure™, 无叠氮钠, 高性能, ≥95%(SDS-PAGE&HPLC)
产品介绍	白细胞介素-18 (IL-18)是一种属于IL-1超家族的细胞因子，由巨噬细胞和其他细胞产生。IL-18通过与IL-18受体结合发挥作用，在脂多糖(LPS)等微生物产物感染后，与IL-12一起诱导细胞介导的免疫。经IL-18刺激后，自然杀伤(NK)细胞和某些T细胞释放另一种重要的细胞因子干扰素-γ (IFN-γ)或ⅱ型干扰素，在激活巨噬细胞或其他细胞中起重要作用。该细胞因子与IL12的结合已被证明可以抑制IL-4依赖性IgE和IgG1的产生，并增强B细胞中IgG2a的产生。IL-18结合蛋白(IL18BP)可与该细胞因子发生特异性相互作用，从而负向调控其生物学活性。 Purity Function >95% (SDS-PAGE&SEC-HPLC) Endotoxin level <10 EU/mg Interleukin-18 (IL-18) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL-4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
生物活性	1、Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 5 - 30 ng/mL. 2、Recombinant Human IL-18 Protein (rp147444) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED50 for this effect is typically 0.27 ng/mL.
内毒素水平	<10 EU/mg
表达系统	E. coli
种属	人(Human)
氨基酸	37-193 aa
序列	YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED
纯度	>95% (SDS-PAGE&SEC-HPLC)
蛋白标签	No tag
无载体	Yes
无动物源	Yes
Accession #	Q14116
来源	重组表达
预测分子量	18.2 kDa
SDS-PAGE	15.4 kDa, under reducing conditions; 15.4 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from sterile 1*PBS, 5% Trehalose, 5% Mannitol.
复溶	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	样品自收到之日起在 -20℃ 至 -80℃ 下可稳定保存 12 个月。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human IL-18 Protein (rp147444)

Recombinant Human IL-18 Protein (rp147444) - Protein Bioactivity
Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED₅₀ for this effect is typically 5 - 30 ng/mL.

Protein Bioactivity: Recombinant Human IL-18 Protein (rp147444)

Recombinant Human IL-18 Protein (rp147444) - Protein Bioactivity
Recombinant Human IL-18 Protein (rp147444) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED₅₀ for this effect is typically 0.27 ng/mL.

SDS-PAGE: Recombinant Human IL-18 Protein (rp147444)

Recombinant Human IL-18 Protein (rp147444) - SDS-PAGE
3 μg/lane of Recombinant Human IL-18 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 15.4 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1202260	分析证书	rp147444
ZJ23F1202261	分析证书	rp147444
ZJ23F1202262	分析证书	rp147444

引用文献

1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]
2. Ushio, S S and 15 more authors.. (1996) Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8666798]
3. Tominaga, K K and 7 more authors.. (2000) IL-12 synergizes with IL-18 or IL-1beta for IFN-gamma production from human T cells.. International immunology, [PMID:10653850]
4. Kato, Zenichiro Z and 14 more authors.. (2003) The structure and binding mode of interleukin-18.. Nature structural biology, [PMID:14528293]
5. Gaggero, Alessia A and 7 more authors.. (2004) A novel isoform of pro-interleukin-18 expressed in ovarian tumors is resistant to caspase-1 and -4 processing.. Oncogene, (30): [PMID:15326478]
6. Jourdan, Tony T and 13 more authors.. (2013) Activation of the Nlrp3 inflammasome in infiltrating macrophages by endocannabinoids mediates beta cell loss in type 2 diabetes.. Nature medicine, [PMID:23955712]
7. Wei, Hui H and 6 more authors.. (2014) Structural basis for the specific recognition of IL-18 by its alpha receptor.. FEBS letters, (3): [PMID:25261253]
8. Tsutsumi, Naotaka N and 12 more authors.. (2014) The structural basis for receptor recognition of human interleukin-18.. Nature communications, (15): [PMID:25500532]

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Recombinant Human IL-18 Protein

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