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Recombinant Human EGF Protein

3篇引用文献 COA COA
  • 表达系统: 毕赤酵母
  • Accession #: P01133
  • 蛋白标签: No tag
  • 生物活性: Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
  • 内毒素水平: <0.01 EU/μg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp185510-50μg
 50μg 期货 Stock Image
rp185510-200μg
 200μg 期货 Stock Image
rp185510-1mg
 1mg 期货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
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点击查看蛋白信息: EGF
Accession#:P01133 (9) Egf (9) Gene ID:1950 (9)
main product photo

基本描述

产品名称 Recombinant Human EGF Protein
别名 重组人表皮生长因子 | 重组人EGF | 重组人EGF蛋白
英文别名 Recombinant Human Epidermal Growth Factor | rhEGF | beta-urogastrone | EGF | epidermal growth factor (beta-urogastrone | epidermal growth factor | hEGF | HOMG4 | pro-epidermal growth factor | URG | Urogastrone
规格或纯度 ActiBioPure™, Bioactive, GMP, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍

pH值：6.5-7.5

人表皮生长因子（EGF）分子量为6.5kD，含有53个氨基酸，分子内有3个二硫键。EGF与位于细胞表面的特异受体EGFR 结合后，引起细胞内一系列的生化反应：提高细胞内的钙离子浓度水平，促进糖酵解的进行，增加蛋白质的合成，并且可以增强EGFR这一类特定基因的表达来促进DNA 的合成和细胞的增殖。

Purity

≥95% SDS-PAGE

Additional sequence information

Mature chain

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Post-translational

O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

pH value: 6.5-7.5

Human epidermal cell growth factor, Mannitol, Sodium dihydrogen monohydrate phosphate, Disodium hydrogen dodecahydrate phosphate, Water 
生物活性 Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
内毒素水平 <0.01 EU/μg
表达系统 毕赤酵母
种属 人(Human)
氨基酸 971-1023 aa
序列 NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
蛋白标签 No tag
无载体 Yes
Accession # P01133
来源 重组表达
预测分子量 6.5kD
SDS-PAGE 7.1 kDa, under reducing conditions.

储存与运输

物理形态 冻干(Lyophilized)
复溶 Reconstitute in sterile water to a concentration of 100-200μg/ml, and further dilute it in other solvents. Upon reconstitution, the product could be stored at 4 ℃ for 2-7 days and used up as soon as possible. For future use, should be stored at -20 ℃.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at -20°C. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS编号和信息 62253-63-8
分子类型 蛋白质

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批号(Lot Number) 证书类型 货号
ZJ24F0505243 分析证书 rp185510
ZJ24F0505244 分析证书 rp185510

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此产品的引用文献

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引用文献

1. Cao Mingbo, Ren Yupeng, Li Yuxuan, Deng Junfeng, Su Xiaorui, Tang Yongchang, Yuan Feng, Deng Haixia, Yang Gaoyuan, He Zhiwei, Liu Bo, Yao Zhicheng, Deng Meihai.  (2023)  Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis.  International Journal of Biological Sciences,  19  (12): (3678-3693).  [PMID:37564197] [10.7150/ijbs.85270]
2. Maoze Guo, Yuqiu Wang, Bingbing Gao, Bingfang He.  (2021)  Shark Tooth-Inspired Microneedle Dressing for Intelligent Wound Management.  ACS Nano,  15  (9): (15316–15327).  [PMID:34533924] [10.1021/acsnano.1c06279]
3. Xiaoli Lu, Shangwen Sun, Na Li, Shuying Hu, Yuyao Pan, Lin Wang, Xuefeng Zhou, Hanbang Chen, Feimin Zhang.  (2024)  Janus sponge/electrospun fibre composite combined with EGF/bFGF/CHX promotes reconstruction in oral tissue regeneration.  COLLOIDS AND SURFACES B-BIOINTERFACES,  243  (114117).  [PMID:39084056] [10.1016/j.colsurfb.2024.114117]

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