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Recombinant Human Legumain/Asparaginyl Endopeptidase Protein

Recombinant Human Legumain/Asparaginyl Endopeptidase Protein

COA

级别和纯度:

无动物源

无载体

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: Q99538
蛋白标签: C-His
生物活性: Testing in progress
内毒素水平: <1.0 EU/μg

有货

库存信息

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货号 (SKU)	包装规格	是否现货
rp184063-10μg	10μg	现货
rp184063-50μg	50μg	现货
rp184063-100μg	100μg	现货
rp184063-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: LGMN

Accession#:Q99538 (2) Gene ID:5641 (2) Legumain/Asparaginyl Endopeptidase (1) LGMN (1)

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基本描述

产品名称	Recombinant Human Legumain/Asparaginyl Endopeptidase Protein
别名	重组人Legumain/Asparaginyl Endopeptidase蛋白 \| 重组人类 Legumain/天冬酰胺内切肽酶蛋白
英文别名	AEP \| Asparaginyl Endopeptidase \| cysteine protease 1 \| Legumain \| LGMN \| LGMN1 \| Protease, cysteine 1 \| protease, cysteine, 1 (legumain) \| PRSC1EC 3.4.22.34
规格或纯度	无动物源, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Protein Function: The Mammalian Legumain, also known as LGMN, also called asparaginyl endopeptidase (AEP), is a cysteine protease belonging to peptidase family C13 with strict specificity for hydrolysis of asparaginyl bonds. Known previously only from plants and invertebrates, Legumain is discovered as a lysosomal endopeptidase in mammals. Mammalian Legumain is a cysteine endopeptidase, inhibited by iodoacetamide and maleimides, but unaffected by compound E64. The Mammalian Legumain is involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Legumain has been observed to be highly expressed in several types of solid tumors. It was demonstrated in membrane-associated vesicles concentrated at the invadopodia of tumor cells and on cell surfaces where it colocalized with integrins. Legumain was demonstrated to activate progelatinase A. Cells overexpressing Legumain possessed increased migratory and invasive activity in vitro and adopted an invasive and metastatic phenotype in vivo, inferring significance of Legumain in tumor invasion and metastasis. Also, Legumain is expressed in both murine and human atherosclerotic lesions. The macrophage-specific expression of Legumain in vivo and the ability of Legumain to induce chemotaxis of monocytes and endothelial cells in vitro suggest that Legumain may play a functional role in atherogenesis.
生物活性	Testing in progress
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-433 aa
序列	MVWKVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHYHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q99538
来源	重组表达
预测分子量	48.5 kDa
SDS-PAGE	56.1 kDa, under reducing conditions; 53.5 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human Legumain/Asparaginyl Endopeptidase Protein (rp184063)

Recombinant Human Legumain/Asparaginyl Endopeptidase (rp184063) - SDS-PAGE
3 μg/lane of Recombinant Human Legumain/Asparaginyl Endopeptidase Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 56.1 kDa under reducing conditions and 53.5 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0809719	分析证书	rp184063
ZJ24F0809720	分析证书	rp184063
ZJ24F0809721	分析证书	rp184063

技术文档和文章

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