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Recombinant Human EphA4 Protein

Recombinant Human EphA4 Protein

级别和纯度:

≥95%(SDS-PAGE)
见COA

表达系统: HEK293
Accession #: P54764
蛋白标签: C-His
生物活性: Immobilized Recombinant Human EphA4 Protein (rp183616) at 2.0 μg/mL can bind Human Recombinant Ephrin-A4 Protein (rp145636) with the EC50 of 8.848 ng/mL.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp183616-10μg	10μg	现货
rp183616-50μg	50μg	现货
rp183616-100μg	100μg	现货
rp183616-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: EPHA4

Accession#: P54764 (1) EPHA4 (1) Gene ID: 2043 (1)

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基本描述

产品名称	Recombinant Human EphA4 Protein
别名	重组人EphA4蛋白 \| 重组人酪氨酸蛋白激酶受体A4蛋白 \| 重组人EphA4蛋白
英文别名	CEK8 \| Cek 8 \| EK8 \| eph receptor a4 \| EPH-like kinase 8 \| EPHA4 \| EPHA4_HUMAN \| Ephrin type-A receptor 4 \| HEK 8 \| hEK8 \| Receptor protein-tyrosine kinase HEK8 \| SEK \| Sek 1 \| TYRO 1 protein tyrosine kinase \| TYRO1 \| Tyrosine-protein kinase receptor SEK
规格或纯度	无动物源, 无载体, 生物活性, ActiBioPure™, ≥95%(SDS-PAGE), 见COA
产品介绍	Protein Function: Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, also plays a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. During development of the cochlear organ of Corti, regulates pillar cell separation by forming a ternary complex with ADAM10 and CADH1 which facilitates the cleavage of CADH1 by ADAM10 and disruption of adherens junctions (By similarity). Phosphorylates CAPRIN1, promoting CAPRIN1-dependent formation of a membraneless compartment (By similarity).
生物活性	Immobilized Recombinant Human EphA4 Protein (rp183616) at 2.0 μg/mL can bind Human Recombinant Ephrin-A4 Protein (rp145636) with the EC50 of 8.848 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-547 aa
序列	MAGIFYFALFSCLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEPSQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRENQFVKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEERSGECQACKIGYYKALSTDATCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTKVSITDLLAHTNYTFEIWAVNGVSKYNPNPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANSTHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P54764
来源	重组表达
预测分子量	59.2 kDa
SDS-PAGE	66.2 kDa, under reducing conditions; 61.1 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
浓度	见COA
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Binding Activity: Recombinant Human EphA4 Protein (rp183616)

Recombinant Human EphA4 Protein (rp183616) - Binding Activity
Immobilized Recombinant Human EphA4 Protein (rp183616) at 2.0 μg/mL can bind Human Recombinant Ephrin-A4 Protein (rp145636) with the EC₅₀ of 8.85 ng/mL.

SDS-PAGE: Recombinant Human EphA4 Protein (rp183616)

Recombinant Human EphA4 Protein (rp183616) - SDS-PAGE
3 μg/lane of Recombinant Human EphA4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 66.2 kDa under reducing conditions and 61.1 kDa under non-reducing conditions

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0809265	分析证书	rp183616
ZJ24F0809266	分析证书	rp183616
ZJ24F0809267	分析证书	rp183616

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