Recombinant Human Cathepsin B Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P07858
蛋白标签: C-His
生物活性: Immobilized Recombinant Human Cathepsin B Protein (rp183609) at 1.0 μg/mL can bind Cathepsin B Mouse mAb (Ab093155) with the EC50 of 163.1 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp183609-10μg	10μg	现货
rp183609-50μg	50μg	现货
rp183609-100μg	100μg	现货
rp183609-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CTSB

Accession#:P07858 (2) Cathepsin B (3) Ctsb (3) Gene ID:1508 (2)

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基本描述

产品名称	Recombinant Human Cathepsin B Protein
别名	重组人Cathepsin B蛋白 \| 重组人组织蛋白酶B蛋白
英文别名	APPS \| Cathepsin B \| Cathepsin B1 \| CPSBamyloid precursor protein secretase \| CTSB \| cysteine protease \| EC 3.4.22 \| EC 3.4.22.1 \| APP secretase
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Protein Function: Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.
生物活性	Immobilized Recombinant Human Cathepsin B Protein (rp183609) at 1.0 μg/mL can bind Cathepsin B Mouse mAb (Ab093155) with the EC50 of 163.1 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-339 aa
序列	MWQLWASLCCLLVLANARSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKIHHHHHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P07858
来源	重组表达
预测分子量	37.3 kDa
SDS-PAGE	40.2-45.0 kDa, under reducing conditions; 37.0-44.0 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

ELISA: Recombinant Human Cathepsin B Protein (rp183609)

Recombinant Human Cathepsin B Protein (rp183609) - ELISA
Immobilized Recombinant Human Cathepsin B Protein (rp183609) at 1.0 μg/mL can bind Cathepsin B Mouse mAb (Ab093155) with the EC50 of 163.1 ng/mL.

SDS-PAGE: Recombinant Human Cathepsin B Protein (rp183609)

Recombinant Human Cathepsin B Protein (rp183609) - SDS-PAGE
3 μg/lane of Recombinant Human Cathepsin B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 40.2-45.0 kDa under reducing conditions and 37.0-44.0 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0708016	分析证书	rp183609
ZJ24F0708017	分析证书	rp183609
ZJ24F0708018	分析证书	rp183609

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