Recombinant Human TrkB Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: Q16620
蛋白标签: C-His
生物活性: Immobilized Recombinant Human TrkB Protein (rp181612) at 1.0 μg/mL can bind Rinat patent anti-TrkB (anti-TrkB) (Ab209857) with the EC50 of 34.2 ng/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp181612-10μg	10μg	现货
rp181612-50μg	50μg	现货
rp181612-100μg	100μg	现货
rp181612-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: NTRK2

Accession#: Q16620 (3) Gene ID: 4915 (3) NTRK2 (3) TrkB (3)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

产品名称	Recombinant Human TrkB Protein
别名	重组人TrkB蛋白 \| 重组人类 TrkB 蛋白
英文别名	BDNF/NT-3 growth factors receptor \| EC 2.7.10 \| EC 2.7.10.1 \| GP145-TrkB \| Neurotrophic tyrosine kinase receptor type 2 \| neurotrophic tyrosine kinase, receptor, type 2 \| NTRK2 \| TrkB tyrosine kinase \| TrkB \| trk-B \| TRKBBDNF-tropomyosine receptor kinase
规格或纯度	无动物源, 无载体, ActiBioPure™, 生物活性, ≥95%(SDS-PAGE)
产品介绍	Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5, and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.
生物活性	Immobilized Recombinant Human TrkB Protein (rp181612) at 1.0 μg/mL can bind Rinat patent anti-TrkB (anti-TrkB) (Ab209857) with the EC50 of 34.2 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-430 aa
序列	MSSWIRWHGPAMARLWGFCWLVVGFWRAAFACPTSCKCSASRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREHHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q16620
来源	重组表达
预测分子量	45.0 kDa
SDS-PAGE	54.2-80.0 kDa, under reducing conditions; 49.9-74.0 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human TrkB Protein (rp181612)

Recombinant Human TrkB Protein (rp181612) - SDS-PAGE
3 μg/lane of Recombinant Human TrkB Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 54.2-80.0 kDa under reducing conditions and 49.9-74.0 kDa under non-reducing conditions.

ELISA: Recombinant Human TrkB Protein (rp181612)

Recombinant Human TrkB Protein (rp181612) - ELISA
Immobilized Recombinant Human TrkB Protein (rp181612) at 1.0 μg/mL can bind Rinat patent anti-TrkB (anti-TrkB) (Ab209857) with the EC50 of 34.2 ng/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0606771	分析证书	rp181612
ZJ24F0606768	分析证书	rp181612
ZJ24F0606769	分析证书	rp181612
ZJ24F0606770	分析证书	rp181612

技术文档和文章

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度