Recombinant Human IL-12/IL-23 p40 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P29460
蛋白标签: C-His
生物活性: Immobilized Recombinant Human IL-12/IL-23 p40 Protein (rp181556) at 1.0 μg/mL can bind Briakinumab (anti-IL-12) (Ab175480) with the EC50 of 17.82 ng/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

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货号 (SKU)	包装规格	是否现货
rp181556-10μg	10μg	期货
rp181556-50μg	50μg	期货
rp181556-100μg	100μg	期货
rp181556-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IL12B

Accession#:P29460 (14) Gene ID:3593 (14) IL-12/IL-23 p40 (1) Il12b (17)

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基本描述

产品名称	Recombinant Human IL-12/IL-23 p40 Protein
别名	重组人IL-12 p40蛋白 \| 重组人IL-23 p40蛋白 \| 重组人 IL-12/IL-23 p40 蛋白
英文别名	CLMF \| CLMF p40 \| CLMF2 \| Cytotoxic lymphocyte maturation factor 40 kDa subunit \| IL12 p40 \| IL-12 p40 \| IL-12 subunit p40 \| IL12B \| IL-12B \| IL-12BNK cell stimulatory factor chain 2 \| interleukin 12, p40 \| interleukin 12B (natural killer cell stimulatory
规格或纯度	ActiBioPure™, 生物活性, 无动物源, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Protein Function: Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.
生物活性	Immobilized Recombinant Human IL-12/IL-23 p40 Protein (rp181556) at 1.0 μg/mL can bind Briakinumab (anti-IL-12) (Ab175480) with the EC50 of 17.82 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-328 aa
序列	MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCSEDLYFQSHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P29460
来源	重组表达
预测分子量	36.4 kDa
SDS-PAGE	40.0-44.3 kDa, under reducing conditions; 36.5-41.0 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

ELISA: Recombinant Human IL-12/IL-23 p40 Protein (rp181556)

Recombinant Human IL-12/IL-23 p40 Protein (rp181556) - ELISA
Immobilized Recombinant Human IL-12/IL-23 p40 Protein (rp181556) at 1.0 μg/mL can bind Briakinumab (anti-IL-12) (Ab175480) with the EC₅₀ of 17.82 ng/mL.

SDS-PAGE: Recombinant Human IL-12/IL-23 p40 Protein (rp181556)

Recombinant Human IL-12/IL-23 p40 Protein (rp181556) - SDS-PAGE
2 μg/lane of Recombinant Human IL-12/IL-23 p40 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 40.0-44.3 kDa under reducing conditions and 36.5-41.0 kDa under non-reducing conditions.

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Recombinant Human IL-12/IL-23 p40 Protein

库存信息

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库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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溶液计算器

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