Recombinant Human IL-6R alpha Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P08887
蛋白标签: C-His
生物活性: 1. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 2.0 μg/mL can bind Recombinant Human IL-6 Protein (rp156005) with the EC50 of 43.35 ng/mL. 2. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Vobarilizumab (anti-IL-6Ra) (Ab175700) with the EC50 of 67.99 ng/mL. 3. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Tocilizumab (anti-IL-6R) (T412004) with the EC50 of 21.05 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp181252-10μg	10μg	现货
rp181252-50μg	50μg	现货
rp181252-100μg	100μg	现货
rp181252-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: IL6R

Accession#:P08887 (9) Gene ID:3570 (9) IL-6R alpha (4) IL6R (10)

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基本描述

产品名称	Recombinant Human IL-6R alpha Protein
别名	重组人IL-6R alpha蛋白 \| 重组人 IL-6R α 蛋白
英文别名	CD126 antigen \| gp80 \| IL 6R \| IL 6R 1 \| IL 6R alpha \| IL-6 receptor alpha chain \| IL-6 receptor subunit alpha \| IL-6R 1 \| IL-6R subunit alpha \| IL-6R-alpha \| IL-6RA \| IL6Q \| Il6r \| CD126 \| IL6RA \| IL6RA_HUMAN \| IL6RQ \| Interleukin 6 receptor \| interleuki
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Protein Function: The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed. A soluble form of the IL-6R alpha has been found in the urine of healthy adult humans. This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6R alpha has been reported. Soluble forms of human and murine IL-6R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6R alpha complex to membrane-bound gp130.
生物活性	1. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 2.0 μg/mL can bind Recombinant Human IL-6 Protein (rp156005) with the EC50 of 43.35 ng/mL. 2. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Vobarilizumab (anti-IL-6Ra) (Ab175700) with the EC50 of 67.99 ng/mL. 3. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Tocilizumab (anti-IL-6R) (T412004) with the EC50 of 21.05 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-365 aa
序列	MLAVGCALLAALLAAPGAALAPRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPPANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQDSSSVPLPHHHHHH
蛋白标签	C-His
N-末端序列	Leu20
无载体	Yes
无动物源	Yes
Accession #	P08887
来源	重组表达
预测分子量	39.4 kDa
SDS-PAGE	60.0-80.8 kDa, under reducing conditions; 60.0-80.7kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Binding Activity: Recombinant Human IL-6R alpha Protein (rp181252)

Recombinant Human IL-6R alpha Protein (rp181252) - Binding Activity
Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 2.0 μg/mL can bind Recombinant Human IL-6 Protein (rp156005) with the EC₅₀ of 43.35 ng/mL.

ELISA: Recombinant Human IL-6R alpha Protein (rp181252)

Recombinant Human IL-6R alpha Protein (rp181252) - ELISA
Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Vobarilizumab (anti-IL-6Ra) (Ab175700) with the EC₅₀ of 67.99 ng/mL.

ELISA: Recombinant Human IL-6R alpha Protein (rp181252)

Recombinant Human IL-6R alpha Protein (rp181252) - ELISA
Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Tocilizumab (anti-IL-6R) (T412004) with the EC₅₀ of 21.05 ng/mL.

SDS-PAGE: Recombinant Human IL-6R alpha Protein (rp181252)

Recombinant Human IL-6R alpha Protein (rp181252) - SDS-PAGE
2 μg/lane of Recombinant Human IL-6R alpha Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 60.0-80.8 kDa under reducing conditions and 60.0-80.7 kDa under non-reducing conditions.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0708065	分析证书	rp181252
ZJ24F0708062	分析证书	rp181252
ZJ24F0708063	分析证书	rp181252
ZJ24F0708064	分析证书	rp181252

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Recombinant Human IL-6R alpha Protein

库存信息

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基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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