Recombinant Human ICAM-1/CD54 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P05362
蛋白标签: C-His
生物活性: Measured by the ability of the immobilized protein to support the adhesion of 5 μg/mL PHA-stimulated human Jurkat T lymphocyte leukemia cell for 2h. The ED50 for this effect is 0.1892 μg/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp181245-10μg	10μg	现货
rp181245-50μg	50μg	现货
rp181245-100μg	100μg	现货
rp181245-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: ICAM1

Accession#:P05362 (5) Gene ID:3383 (6) ICAM-1/CD54 (1) ICAM1 (7)

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基本描述

产品名称	Recombinant Human ICAM-1/CD54 Protein
别名	重组人ICAM-1/CD54蛋白 \| 重组人类 ICAM-1/CD54 蛋白
英文别名	CD54 antigen \| CD54 \| cell surface glycoprotein P3.58 \| human rhinovirus receptor \| ICAM1 \| ICAM-1 \| intercellular adhesion molecule 1 (CD54), human rhinovirus receptor \| intercellular adhesion molecule 1 \| Major group rhinovirus receptor \| P3.58 \| BB2
规格或纯度	Animal Free, Carrier Free, ActiBioPure™, Bioactive, High performance, ≥95%(SDS-PAGE)
产品介绍	Protein Function: Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
生物活性	Measured by the ability of the immobilized protein to support the adhesion of 5 μg/mL PHA-stimulated human Jurkat T lymphocyte leukemia cell for 2h. The ED50 for this effect is 0.1892 μg/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-480 aa
序列	MAPSSPRPALPALLVLLGALFPGPGNAQTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSPRYEHHHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P05362
来源	重组表达
预测分子量	50.6 kDa
SDS-PAGE	62.6-88.8 kDa, under reducing conditions; 58.1-81.5 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human ICAM-1/CD54 Protein (rp181245)

Recombinant Human ICAM-1/CD54 Protein (rp181245) - Protein Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of 5 μg/mL PHA-stimulated human Jurkat T lymphocyte leukemia cell for 2h. The ED₅₀ for this effect is 0.1892 μg/mL.

SDS-PAGE: Recombinant Human ICAM-1/CD54 Protein (rp181245)

Recombinant Human ICAM-1/CD54 Protein (rp181245) - SDS-PAGE
3 μg/lane of Recombinant Human ICAM-1/CD54 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 62.6-88.8 kDa under reducing conditions and 58.1-81.5 kDa under non-reducing conditions.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0707696	分析证书	rp181245
ZJ24F0707697	分析证书	rp181245
ZJ24F0707698	分析证书	rp181245
ZJ24F0707701	分析证书	rp181245

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