Recombinant Human Mesothelin Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: Q13421
蛋白标签: C-His
生物活性: Measured by its binding ability in a functional ELISA. Human Mesothelin protein (rp176301) at 1 μg/mL can bind Human Osteopontin ( rp149597) with the EC50 of 30.0 ng/mL.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp176301-10μg	10μg	现货
rp176301-50μg	50μg	现货
rp176301-100μg	100μg	现货
rp176301-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: MSLN

Accession#:Q13421 (6) Gene ID:10232 (6) Mesothelin (7) Msln (7)

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基本描述

产品名称	Recombinant Human Mesothelin Protein
别名	重组人Mesothelin蛋白 \| 重组人间皮蛋白 \| 重组人间皮素蛋白
英文别名	CAK 1 \| CAK1 \| CAK1 antigen \| cleaved form \| Megakaryocyte potentiating factor \| Mesothelin \| Mesothelin isoform 1 precursor \| MPF \| MPFSMRP \| MSLN \| MSLN_HUMAN \| Pre pro megakaryocyte potentiating factor \| Pre-pro-megakaryocyte-potentiating factor \| SMR
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Mesothelin (MSLN), also known as CAK1 and ERC, is a glycosylated cell-surface antigen present on normal mesothelial cells and over-expressed in several human tumors. The mesothelin gene encodes a ~70 kDa precursor protein that is cleaved at a dibasic proteolytic site into a 40 kDa membrane-bound protein termed MSLN and a 31 kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Cleaved, human MSLN remains attached to the cell surface via a GPI linkage and shares 58% amino acid sequence identity with mouse and rat MSLN. In human, alternate splicing generates additional MSLN isoforms that have either an eight amino acid insertion following Ser408 or a substituted C‑terminal region with no GPI anchor. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is up‑regulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression. It is co‑expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion. A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions.
生物活性	Measured by its binding ability in a functional ELISA. Human Mesothelin protein (rp176301) at 1 μg/mL can bind Human Osteopontin ( rp149597) with the EC50 of 30.0 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-286 aa
序列	MALPTARPLLGSCGTPALGSLLFLLFSLGWVQPSRTLAGETGQEAAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRLLPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSWRQPERhhhhhh
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q13421
来源	重组表达
预测分子量	27.5 kDa
SDS-PAGE	30.3 kDa, reducing conditions; 30.3 kDa, non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

ELISA: Recombinant Human Mesothelin Protein (rp176301)

Recombinant Human Mesothelin Protein (rp176301) - ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Human Osteopontin (rp149597) is immobilized at 0.5 µg/mL, biotinylated Recombinant Human Mesothelin (rp176301) binds with an ED₅₀ of 30.73 ng/mL.

SDS-PAGE: Recombinant Human Mesothelin Protein (rp176301)

Recombinant Human Mesothelin Protein (rp176301) - SDS-PAGE
3 μg/lane of Recombinant Mesothelin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.3 kDa.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0303047	分析证书	rp176301
ZJ24F0303044	分析证书	rp176301
ZJ24F0303045	分析证书	rp176301
ZJ24F0303046	分析证书	rp176301

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