Recombinant Human Glypican 3 Protein

1篇引用文献

级别和纯度:

≥90%(SDS-PAGE)

表达系统: HEK293
Accession #: P51654-1
蛋白标签: C-His
生物活性: Immobilized Recombinant Human Glypican 3 Protein (rp176299) at 4.0 μg/mL can bind Recombinant Human FGF basic Protein (rp156010) with the EC50 of 441.4 ng/mL. Immobilized Recombinant Human FGF basic Protein (rp156010) at 1.0 μg/mL can bind Recombinant Human Glypican 3 Protein (rp176299) with the EC50 of 7.4 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp176299-10μg	10μg	现货
rp176299-50μg	50μg	现货
rp176299-100μg	100μg	现货
rp176299-1mg	1mg	期货

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点击查看蛋白信息: GPC3

Accession#:P51654 (2) Gene ID:2719 (2) Glypican 3 (2) GPC3 (2)

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基本描述

产品名称	Recombinant Human Glypican 3 Protein
别名	重组人Glypican 3蛋白 \| 重组人类磷脂酰肌醇蛋白聚糖 3 蛋白
英文别名	DGSX \| Glypican 3 \| glypican proteoglycan 3 \| glypican-3 \| GPC3 \| GTR2-2 \| heparan sulphate proteoglycan \| Intestinal protein OCI-5 \| MXR7 \| OCI5 \| OCI-5 \| secreted glypican-3 \| SGB \| SGBS \| SGBS1SDYS
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥90%(SDS-PAGE)
产品介绍	Protein Function: Glypican-3, also known as Intestinal protein OCI-5, GPC3, and OCI5, is a member of the glypican family. It belongs to the glypican family and is highly expressed in the lung, liver, and kidney. It is a heparan sulfate proteoglycan, which is overexpressed in various neoplasms such as hepatocellular carcinoma, malignant melanoma, and testicular yolk sac tumor, and plays an important role in cell growth and differentiation. GPC3 function is tissue-dependent. In some tissues, GPC3 acts as a tumor suppressor gene, whereas in others, it acts as an oncofetal protein. Studies have shown that GPC3 is a reliable marker for hepatocellular carcinoma. The sensitivity and specificity exceed both alpha-fetoprotein and hepatocyte-paraffin1. GPC3 immunohistochemistry can aid in the differentiation of testicular germ cell tumors, being expressed in all yolk sac tumors but not in seminomas. GPC3 expression has also been identified in some squamous cell carcinomas of the lung and clear cell carcinomas of the ovary. The role of GPC3 in melanomas is still controversial. Thus, Glypican-3 is currently regarded as a tumor marker and potential target for immunotherapy.
生物活性	Immobilized Recombinant Human Glypican 3 Protein (rp176299) at 4.0 μg/mL can bind Recombinant Human FGF basic Protein (rp156010) with the EC50 of 441.4 ng/mL. Immobilized Recombinant Human FGF basic Protein (rp156010) at 1.0 μg/mL can bind Recombinant Human Glypican 3 Protein (rp176299) with the EC50 of 7.4 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-559 aa
序列	MAGTVRTACLVVAMLLSLDFPGQAQPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P51654-1
来源	重组表达
预测分子量	61.5 kDa
SDS-PAGE	30.0 kDa & 44.2 kDa & 66.2 kDa, under reducing conditions; 60.0 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Binding Activity: Recombinant Human Glypican 3 Protein (rp176299)

Recombinant Human Glypican 3 Protein (rp176299) - Binding Activity
Immobilized Recombinant Human FGF basic Protein (rp156010) at 1.0 μg/mL can bind Recombinant Human Glypican 3 Protein (rp176299) with the EC50 of 7.4 ng/mL.

Binding Activity: Recombinant Human Glypican 3 Protein (rp176299)

Recombinant Human Glypican 3 Protein (rp176299) - Binding Activity
Immobilized Recombinant Human Glypican 3 Protein (rp176299) at 4.0 μg/mL can bind Recombinant Human FGF basic Protein (rp156010) with the EC50 of 441.4 ng/mL.

SDS-PAGE: Recombinant Human Glypican 3 Protein (rp176299)

Recombinant Human Glypican 3 Protein (rp176299) - SDS-PAGE
2 μg/lane of Recombinant Human Glypican 3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.0 kDa & 44.2 kDa & 66.2 kDa under reducing conditions and 60.0 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0707679	分析证书	rp176299
ZJ24F0707680	分析证书	rp176299
ZJ24F0707681	分析证书	rp176299

技术文档和文章

引用文献

1. Xiaohong Tan, Wenzhan Li, Wei He, Tingting Yu, Bingbing Wan, Yong Huang, Jintao Liang, Guiyin Li. (2024) A highly sensitive sandwich-type electrochemical sensor for detection glypican-3 based on H-rGO-CMC@Pt NPs and aptamers. MICROCHEMICAL JOURNAL, 207 (111747). [10.1016/j.microc.2024.111747]

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Recombinant Human Glypican 3 Protein

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基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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引用文献

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