Recombinant Human B7-H3(4Ig)/B7-H3b Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: Q5ZPR3
蛋白标签: C-His
生物活性: 1、Measured by its binding ability in a functional ELISA. Immobilized CD276 mouse mAb at 1.0 μg/mL can bind Recombinant Human B7-H3 (4Ig)/B7-H3b Protein (rp176244) with the EC50 of 51.95 ng/mL. 2、Measured by its binding ability in a functional ELISA. Immobilized human Human B7-H3 (4Ig)/B7-H3b (rp176244) at 1.0 μg/mL can bind CD276 mouse mAb with the EC50 of 1.86 ng/mL.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp176244-10μg	10μg	现货
rp176244-50μg	50μg	现货
rp176244-100μg	100μg	现货
rp176244-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CD276

Accession#:Q5ZPR3 (8) B7-H3 (4Ig) (1) B7-H3b (1) CD276 (9) Gene ID:80381 (8)

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基本描述

产品名称	Recombinant Human B7-H3(4Ig)/B7-H3b Protein
别名	重组人B7-H3 (4Ig)/B7-H3b蛋白 \| 重组人 B7-H3(4Ig)/B7-H3b 蛋白
英文别名	4Ig-B7-H3 \| B7 homolog 3 \| B7-H3 \| Costimulatory molecule \| 4Ig B7 H3 \| 4Ig-B7-H3 \| B7H34Ig-B7-H3 \| B7-H3B7 homolog 3 \| AU016588 \| B7 H3 \| B7 homolog 3 \| B7-H3 \| B7H3 \| B7RP-2 \| CD_antigen=CD276 \| CD276 \| CD276 antigen \| CD276 molecule \| CD276_HUMAN \| Cos
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining Description: Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Among the family members there is about 20 - 40% amino acid (aa) sequence identity. B7-H3 was initially reported to be a 316 aa type I transmembrane precursor protein that contained a signal sequence, an extracellular region with one V‑type and one C‑type Ig domain, a transmembrane segment and a short cytoplasmic tail. Subsequent studies have identified a second 110 kDa form whose precursor is 534 aa in length. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern. It would appear that the human 4Ig form is the principal, if not the only form of B7-H3. Its precursor contains a 26 aa signal sequence, a 435 aa extracellular region, a 31 aa transmembrane domain, and a 42 aa cytoplasmic tail. The four Ig-like domains alternate between V‑type and C‑type, and apparently are the consequence of a V‑C type tandem duplication. B7-H3b is expressed on dendritic cells as well as activated T, B and NK cells. The mouse gene differs from that of human in that it cannot code for four Ig-like domains; only a V‑type:C‑type pair. Human B7-H3b binding to an undefined receptor has shown to be inhibitory to NK cell illing and cytokine release. It also seems to be required for late stage osteoblast differentiation.
生物活性	1、Measured by its binding ability in a functional ELISA. Immobilized CD276 mouse mAb at 1.0 μg/mL can bind Recombinant Human B7-H3 (4Ig)/B7-H3b Protein (rp176244) with the EC50 of 51.95 ng/mL. 2、Measured by its binding ability in a functional ELISA. Immobilized human Human B7-H3 (4Ig)/B7-H3b (rp176244) at 1.0 μg/mL can bind CD276 mouse mAb with the EC50 of 1.86 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	29-461 aa
序列	LEVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYQGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSILRVVLGANGTYSCLVRNPVLQQDAHSSVTITPQRSPTGAVEVQVPEDPVVALVGTDATLRCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFTEGRDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQPMTHHHHHHHHHH
纯度	>95% (SDS-PAGE)
蛋白标签	C-His
N-末端序列	Gly27
无载体	Yes
无动物源	Yes
Accession #	Q5ZPR3
来源	重组表达
预测分子量	47.9 kDa
SDS-PAGE	63.2-82.3 kDa, under reducing conditions; 58.6-73.9 kDa, under non-reducing condition

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

ELISA: Recombinant Human B7-H3(4Ig)/B7-H3b Protein (rp176244)

ELISA: Recombinant Human B7-H3(4Ig)/B7-H3b Protein (rp176244)
Measured by its binding ability in a functional ELISA. Immobilized CD276 mouse mAb at 1.0 μg/mL can bind Recombinant Human B7-H3 (4Ig)/B7-H3b Protein (rp176244) with the EC₅₀ of 51.95 ng/mL.

ELISA: Recombinant Human B7-H3(4Ig)/B7-H3b Protein (rp176244)

Recombinant Human B7-H3(4Ig)/B7-H3b Protein (rp176244) - ELISA
Measured by its binding ability in a functional ELISA. Immobilized human Human B7-H3 (4Ig)/B7-H3b (rp176244) at 1.0 μg/mL can bind CD276 mouse mAb with the EC₅₀ of 1.86 ng/mL.

SDS-PAGE: Recombinant Human B7-H3(4Ig)/B7-H3b Protein (rp176244)

Recombinant Human B7-H3(4Ig)/B7-H3b Protein (rp176244) - SDS-PAGE
3 μg/lane of Recombinant Human B7-H3(4Ig)/B7-H3b Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 63.2 - 82.3 kDa under reducing conditions and 58.6 - 73.9 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0202914	分析证书	rp176244
ZJ24F0202912	分析证书	rp176244
ZJ24F0202913	分析证书	rp176244

技术文档和文章

引用文献

1. Loo D, Alderson RF, Chen FZ, Huang L, Zhang W, Gorlatov S, Burke S, Ciccarone V, Li H, Yang Y et al.. (2012) Development of an Fc-enhanced anti-B7-H3 monoclonal antibody with potent antitumor activity.. Clin Cancer Res, 18 (14): (3834-45). [PMID:22615450]
2. Chapoval AI, Ni J, Lau JS, Wilcox RA, Flies DB, Liu D, Dong H, Sica GL, Zhu G, Tamada K et al.. (2001) B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production.. Nat Immunol, 2 (3): (269-74). [PMID:11224528]
3. Castriconi R, Dondero A, Augugliaro R, Cantoni C, Carnemolla B, Sementa AR, Negri F, Conte R, Corrias MV, Moretta L et al.. (2004) Identification of 4Ig-B7-H3 as a neuroblastoma-associated molecule that exerts a protective role from an NK cell-mediated lysis.. Proc Natl Acad Sci USA, 101 (34): (12640-5). [PMID:15314238]
4. Ling, Vincent V and 6 more authors.. (2003) Duplication of primate and rodent B7-H3 immunoglobulin V- and C-like domains: divergent history of functional redundancy and exon loss.. Genomics, [PMID:12906861]
5. Steinberger, Peter P and 9 more authors.. (2004) Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:14764704]
6. Wang, Liqing L and 7 more authors.. (2005) B7-H3 promotes acute and chronic allograft rejection.. European journal of immunology, [PMID:15682454]
7. Kim, Jean J and 8 more authors.. (2005) Constitutive and inducible expression of b7 family of ligands by human airway epithelial cells.. American journal of respiratory cell and molecular biology, [PMID:15961727]
8. Petroff, Margaret G MG, Kharatyan, Elza E, Torry, Donald S DS and Holets, Lesya L.. (2005) The immunomodulatory proteins B7-DC, B7-H2, and B7-H3 are differentially expressed across gestation in the human placenta.. The American journal of pathology, [PMID:16049332]
9. Zhang, Guangbo G, Dong, Qiuming Q, Xu, Ying Y, Yu, Gehua G and Zhang, Xueguang X.. (2005) B7-H3: another molecule marker for Mo-DCs?. Cellular & molecular immunology, [PMID:16274630]
10. Otsuki, Tetsuji T and 24 more authors.. (2005) Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:16303743]
11. Hashiguchi, Masaaki M and 5 more authors.. (2008) Triggering receptor expressed on myeloid cell-like transcript 2 (TLT-2) is a counter-receptor for B7-H3 and enhances T cell responses.. Proceedings of the National Academy of Sciences of the United States of America, (29): [PMID:18650384]

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